<b>Background and Objective:</b> <i>Oroxylum indicum</i>, a plant commonly used in traditional medicine to address various human ailments, has recently gained attention as a promising candidate in this regard due to its rich phytochemical composition and potential antibacterial properties. This study was undertaken to evaluate the antibacterial efficacy of <i>O. indicum</i> extracts, specifically from its leaves and stems, against antibiotic-resistant bacteria. <b>Materials and Methods:</b> Stems and leaves of <i>O. indicum</i> were extracted using ethanol, hexane and dichloromethane. The antibacterial activity of the extracts was initially evaluated through the disc diffusion method, while the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using the broth microdilution method. The inhibition zone diameters (mm) were statistically analyzed using Duncan's Multiple Range Test (DMRT) in SAS software at a p-value threshold of <0.05. <b>Results:</b> The ethanolic stem and leaf extracts of <i>Oroxylum</i> <i>indicum</i> produced the largest inhibition zone of 11 mm against <i>Proteus mirabilis</i>, highlighting a significant antibacterial response. Further analysis showed that the lowest minimum inhibitory concentration (MIC) was recorded at 1.56 mg/mL in these ethanolic extracts, effective against both <i>Acinetobacter baumannii</i> and <i>Proteus mirabilis</i>. The minimum bactericidal concentration (MBC) was observed at 6.25 mg/mL for these bacteria, indicating a potent bactericidal effect. <b>Conclusion:</b> This study represents the first documented instance of <i>O. indicum</i> extracts effectively targeting antibiotic-resistant bacteria, thereby underscoring their potential as a foundation for developing new antibiotics. The findings pave the way for further research and development, contributing to the critical pursuit of novel therapeutic agents to combat resistant bacterial infections.
