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Clinical and experimental vaccine research
Jan, 2025;14 (1): 86-100.

Discovery of novel vaccine candidates based on the immunogenic epitopes derived from membrane proteins.

Seyyed Amir Hosseini, Saman Hashemi, Davood Siamian, Ali Asghari, Mohammad Fathollahzadeh, Hamidreza Majidiani, Iman Shahraki, Mohamad Hosein Safari
Author Information
  1. Seyyed Amir Hosseini: Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. ORCID
  2. Saman Hashemi: MS.C. in Poultry Nutrition, College of Veterinary, Razi University, Kermanshah, Iran. ORCID
  3. Davood Siamian: Department of Biology, Faculty of Basic Science, Islamic Azad University, Tonekabon Branch, Mazandaran, Iran. ORCID
  4. Ali Asghari: Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. ORCID
  5. Mohammad Fathollahzadeh: Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ORCID
  6. Hamidreza Majidiani: Healthy Aging Research Centre, Neyshabur University of Medical Sciences, Neyshabur, Iran. ORCID
  7. Iman Shahraki: Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran. ORCID
  8. Mohamad Hosein Safari: Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. ORCID
PMID: 39927227 DOI: 10.7774/cevr.2025.14.e4

Abstract

Purpose: Due to the widespread distribution and importance of infection as a parasitic zoonosis, multi-epitope vaccine design was implemented using a set of immunodominant epitopes screened out of a wide scope of membrane proteins.
Materials and Methods: On this basis, 5 vaccine candidates were created using linkers ([GGGGS], KK, AAY, GPGPG, GDGDG, EAAAK) and adjuvants (RS-09 peptide, resuscitation-promoting factor E [RpfE] and 50S ribosomal protein, human interferon [IFN]-��).
Results: Polytopes with RS-09 alone (Toxo-App) and with IFN-�� (Toxo-Apfn), and one with 50S ribosomal protein (Toxo-Ribos) showed the highest immunogenicity during prediction, and their 3-dimensional structure was refined. Protein-protein docking and molecular dynamics simulation analysis was done between the Toxo-App and human toll-like receptor (TLR)-4, rendering a stable connection. Codon optimization and cloning was done ultimately for the selected vaccine candidate.
Conclusion: In conclusion, potent multi-epitope vaccine candidates were designed against toxoplasmosis using a diverse set of techniques, while further wet experiments are recommended.

Keywords

Immunoinformatics Toxoplasma gondii Vaccination Vaccine design

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Journal Article

Word Cloud

Created with Highcharts 10.0.0vaccineusingcandidatesmulti-epitopedesignsetepitopesmembraneproteinsRS-0950SribosomalproteinhumanToxo-AppdonePurpose:DuewidespreaddistributionimportanceinfectionparasiticzoonosisimplementedimmunodominantscreenedwidescopeMaterialsMethods:basis5createdlinkers[GGGGS]KKAAYGPGPGGDGDGEAAAKadjuvantspeptideresuscitation-promotingfactorE[RpfE]interferon[IFN]-��Results:PolytopesaloneIFN-��Toxo-ApfnoneToxo-Ribosshowedhighestimmunogenicityprediction3-dimensionalstructurerefinedProtein-proteindockingmoleculardynamicssimulationanalysistoll-likereceptorTLR-4renderingstableconnectionCodonoptimizationcloningultimatelyselectedcandidateConclusion:conclusionpotentdesignedtoxoplasmosisdiversetechniqueswetexperimentsrecommendedDiscoverynovelbasedimmunogenicderivedImmunoinformaticsToxoplasmagondiiVaccinationVaccine

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