Rapid spread of the emerging cosmopolitan genotype of dengue virus serotype 2, and expansion of dengue virus serotype 1 genotype V in Peru.
Henri Bailon, V��ctor Jimenez, Marco Galarza, Princesa Medrano, Orson Mestanza, Dana Figueroa, Wendy Lizarraga, Iris Silva, Luren Sevilla, Ver��nica Hurtado, Vanessa Izarra, Carlos Padilla, Luis Barcena, Omar Caceres, Susy Merino, Adolfo Marcelo, Nora Ruiz, Hapuarachchige Chanditha Hapuarachchii, C��sar Cabezas S��nchez, Mar��a P Garc��a
Author Information
Henri Bailon: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
V��ctor Jimenez: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Marco Galarza: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Princesa Medrano: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Orson Mestanza: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Dana Figueroa: Laboratorio Nacional de Referencia de Enfermedades Metax��nicas y Zoonosis Virales, CNSP-INS, Lima, Per��. ORCID
Wendy Lizarraga: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Iris Silva: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Luren Sevilla: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Ver��nica Hurtado: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Vanessa Izarra: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Carlos Padilla: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Luis Barcena: ��rea de Innovaci��n y Desarrollo (Equipo de Vigilancia Gen��mica), Centro Nacional de Salud P��blica (CNSP), Instituto Nacional de Salud (INS), Lima, Per��. ORCID
Omar Caceres: Laboratorio Nacional de Referencia de Virus Inmunoprevenibles, CNSP-INS, Lima, Per��. ORCID
Susy Merino: Laboratorio Nacional de Referencia de Enfermedades Metax��nicas y Zoonosis Virales, CNSP-INS, Lima, Per��. ORCID
Adolfo Marcelo: Laboratorio Nacional de Referencia de Enfermedades Metax��nicas y Zoonosis Virales, CNSP-INS, Lima, Per��. ORCID
Nora Ruiz: Laboratorio Nacional de Referencia de Virus Inmunoprevenibles, CNSP-INS, Lima, Per��. ORCID
Hapuarachchige Chanditha Hapuarachchii: Divisi��n de Microbiolog��a y Epidemiolog��a Molecular, Instituto de Salud Medioambiental, Agencia Nacional de Medio Ambiente, Singapur. ORCID
C��sar Cabezas S��nchez: Instituto Nacional de Salud, Lima, Per��. ORCID
Mar��a P Garc��a: Laboratorio Nacional de Referencia de Enfermedades Metax��nicas y Zoonosis Virales, CNSP-INS, Lima, Per��. ORCID
BACKGROUND: Motivation for the study. This study aimed to analyze the prevalence and distribution of the V genotypes of dengue virus type 1 (DENV-1) and the cosmopolitan genotype DENV-2 in Peru, in order to understand their expansion in different regions; since these viruses can cause important outbreaks in the country. Main findings. A rapid spread and wide distribution of the cosmopolitan DENV-2 genotype was reported in Peru in 2022, following its initial introduction in 2019. DENV-1 genotype V, present in Peru since the 1990s, has now expanded to new Peruvian regions, after previously being restricted to rural and jungle areas in the north of the country. Implications. The results highlight the urgent need to strengthen epidemiological and genomic surveillance systems to monitor and control the spread of these genotypes in Peru, allowing a more rapid response and controlling their potential spread to other regions of the Americas. OBJECTIVES.: This study aimed to evaluate the prevalence and distribution of dengue virus genotype V serotype 1 (DENV-1) and cosmopolitan genotype serotype 2 (DENV-2) in Peru between 2019 and 2022. MATERIALS AND METHODS.: The envelope (E) gene region was amplified from 79 serum samples by polymerase chain reaction (PCR) and sequenced by next-generation sequencing (NGS) technology. The obtained sequences were subsequently analyzed with bioinformatics tools. RESULTS.: The study generated envelope gene sequences of DENV-1 and DENV-2 serotypes. Our study revealed a rapid dispersal and wide distribution of the cosmopolitan DENV-2 genotype in several regions of Peru in 2022, as well as the spread of DENV-1 genotype V to new Peruvian regions, along with the cosmopolitan DENV-2 genotype. CONCLUSIONS.: Our findings suggest the urgent need to strengthen epidemiological and genomic surveillance systems to understand and control the spread of circulating DENV genotypes in Peru. This will allow a more rapid response, as well as the monitoring of its potential dissemination to other countries in the Americas.
