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Animal genetics
Feb, 2025;56 (1): e70002.

Characterization of DNA methylation profile of the entire CpG island spanning the 5' untranslated region to intron 1 of the Oct4/POU5F1 gene in bovine gametes, embryos, and somatic cells.

Amanda Oliveira Moura, Thainara Christie Ferreira Silva, Alexandre Rodrigues Caetano, Nayara Ribeiro Kussano, Margot Alves Nunes Dode, Maur��cio Machaim Franco
Author Information
  1. Amanda Oliveira Moura: School of Veterinary Medicine, Federal University of Uberl��ndia (FAMEV-UFU), Uberl��ndia, Minas Gerais, Brazil. ORCID
  2. Thainara Christie Ferreira Silva: EMBRAPA Genetic Resources and Biotechnology, Bras��lia, Distrito Federal, Brazil.
  3. Alexandre Rodrigues Caetano: EMBRAPA Genetic Resources and Biotechnology, Bras��lia, Distrito Federal, Brazil.
  4. Nayara Ribeiro Kussano: EMBRAPA Genetic Resources and Biotechnology, Bras��lia, Distrito Federal, Brazil.
  5. Margot Alves Nunes Dode: EMBRAPA Genetic Resources and Biotechnology, Bras��lia, Distrito Federal, Brazil.
  6. Maur��cio Machaim Franco: School of Veterinary Medicine, Federal University of Uberl��ndia (FAMEV-UFU), Uberl��ndia, Minas Gerais, Brazil. ORCID
PMID: 39953930 DOI: 10.1111/age.70002

Abstract

Stem cells are undifferentiated cells that exhibit a bivalent chromatin state that determines their fate. These cells have potential applications in human and animal health and livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome is the main obstacle to correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes in the somatic genome is a promising strategy to contribute to more efficient epigenetic reprogramming, improving this technique. Recently, epigenome editing has emerged as a new generation of clustered regularly interspaced short palindromic repeats-clustered regularly interspaced short palindromic repeats-associated protein 9 technology with the aim of modifying the cellular epigenome to turn genes on or off without modifying DNA. Here, we characterize the DNA methylation profile of the CpG island spanning the 5' untranslated region to intron 1 of the bovine octamer-binding transcription factor (Oct4) gene in gametes, embryos, and fibroblasts. DNA methylation patterns were categorized into three levels: low (0%-20%), moderate (21%-50%), and high (51%-100%). Sperm and embryos showed a hypomethylation pattern, whereas oocytes exhibited a hypo- to moderate methylation pattern. Fetal and adult skin fibroblasts were hypomethylated and moderately methylated, respectively. These results are essential for future studies aimed at manipulating the expression of Oct4. Thus, epigenome editing can be used to turn on the Oct4 in somatic cells to generate induced pluripotent stem cells. This strategy could potentially convert a fully differentiated cell into a cell with certain degree of pluripotency, facilitating nuclear reprogramming by the enucleated oocyte and improving cloning success rates.

Keywords

Oct4 DNA methylation bovine epigenetics

Associated Data

RefSeq | NM_174580

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Grants

  1. /Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico
  2. /Funda����o de Apoio �� Pesquisa do Distrito Federal - FAP DF

MeSH Term

Animals
DNA Methylation
Cattle
Octamer Transcription Factor-3
CpG Islands
Male
Female
Spermatozoa
Introns
Oocytes
Embryo, Mammalian
5' Untranslated Regions
Fibroblasts
Epigenesis, Genetic

Chemicals

Octamer Transcription Factor-3
5' Untranslated Regions
Journal Article

Word Cloud

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