High-protein diet based on whey protein isolate preserves muscle mass and its association with resistance training attenuates glomerular hypertrophy in rats.
Eudes Thiago Pereira ��vila, Thiago da Rosa Lima, Paula Caroline de Almeida, Geovane Jos�� Tolazzi, Let��cia Pereira Cavalcante, Diego Luiz Doneda, Gabrielle Barcelo Paes, Roberto Carlos Viera Junior, Am��lcar Sabino Damazo, Fabr��cio Azevedo Voltarelli
Author Information
Eudes Thiago Pereira ��vila: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil; State University of Mato Grosso, C��ceres Mato Grosso, Brazil; Fasipe College Cuiab��, Cuiab�� Mato Grosso, Brazil.
Thiago da Rosa Lima: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil; University Center of Varzea Grande, Varzea Grande Mato Grosso, Brazil.
Paula Caroline de Almeida: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil.
Geovane Jos�� Tolazzi: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil.
Let��cia Pereira Cavalcante: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil.
Diego Luiz Doneda: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil.
Gabrielle Barcelo Paes: Postgraduate Program in Nutrition, Food and Metabolism, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil.
Roberto Carlos Viera Junior: State University of Mato Grosso, C��ceres Mato Grosso, Brazil.
Am��lcar Sabino Damazo: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil.
Fabr��cio Azevedo Voltarelli: Postgraduate Program in Health Sciences, Federal University of Mato Grosso, Cuiab��, Mato Grosso, Brazil. Electronic address: voltarellifa@gmail.com.
OBJECTIVE: To evaluate the effect of administering a high-protein diet (HP) based on whey protein isolate (WPI) associated with ST in rats. METHODS: Twenty-two Wistar rats were fed a diet containing either 14% WPI (normoproteic) or 35% WPI (high protein) and were subjected to stair training or kept sedentary for 12 weeks. SN = sedentary normoproteic; SH = sedentary high protein; TN = trained normoproteic; TH = trained high protein. RESULTS: HP decreases food intake (P < 0.0001). The SH group showed a significant reduction in plasma triglycerides (P = 0.03). Quadriceps weight was greatest in TH, followed by SH and TN (P < 0.0001). Kidney weight was greater in TH, followed by SH and TN, and was greater than in SN (P < 0.0001). Urea levels were lowest in the SN group (P < 0.001). The urinary space was larger in the TH and SH groups. HP and ST increased the cross-sectional area (CSA) of the gastrocnemius (P < 0.0001) and quadriceps (P < 0.0001) muscles. Hepatic glycogen deposits were highest in the SH group (P < 0.0001). CONCLUSIONS: HP with 35% whey promoted satiety and increased hepatic glycogen content without affecting glycemia. Its combination with ST was more efficient in increasing muscle hypertrophy, altering plasma urea levels, and enlarging the urinary space. These findings may be related to the adaptive process of renal physiology stimulated by HP.
