Morphological and Molecular Characterization of a Novel Fungal-feeding Stem Nematode n. sp. (Tylenchida: Anguinidae) from Intercepted Samples.

Che-Chang Liang, Pei-Chen Chen
Author Information
  1. Che-Chang Liang: Department of Plant Pathology, College of Agriculture and Natural Resources, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung City 402, Taiwan (R.O.C.).
  2. Pei-Chen Chen: Department of Plant Pathology, College of Agriculture and Natural Resources, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung City 402, Taiwan (R.O.C.).

Abstract

A new species of the genus , n. sp., collected from coconut fiber used as growing media for staghorn ferns and intercepted during import quarantine, is described and illustrated herein based on morphological and molecular studies. The new species is characterized by a body length of 728 (612-846) μm and 641 (511-720) μm in female and male, respectively, delicate stylet 8.0 (7.4-8.4) μm long, six lines in the lateral field, median bulb of esophagus well-developed, muscular with crescentic valve, post-vulval uterine sac well-developed, 36 (22-52) μm long, female tail elongate-conoid with finely rounded terminus. The results of phylogenetic analyses based on sequences of the D2D3 expansion region of 28S rRNA and ITS confirmed the close molecular relationship between n. sp., and other species such as , , , , and . n. sp. was successfully reared on the and . However, n. sp. did not reproduce when culturing on , , , , nor on callused carrot disks, and alfalfa seedlings.

Keywords

References

  1. Mol Genet Genomics. 2019 Oct;294(5):1211-1218 [PMID: 31076860]
  2. J Nematol. 1983 Jan;15(1):142-3 [PMID: 19295781]
  3. Environ Pollut. 2010 May;158(5):1741-7 [PMID: 20004050]
  4. J Nematol. 1990 Jul;22(3):399-406 [PMID: 19287736]
  5. J Nematol. 1970 Oct;2(4):375-92 [PMID: 19322328]
  6. Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1945-58 [PMID: 16214752]
  7. Phytopathology. 2005 Nov;95(11):1308-15 [PMID: 18943362]
  8. J Nematol. 1971 Jan;3(1):79-84 [PMID: 19322344]
  9. J Helminthol. 2016 Mar;90(2):152-65 [PMID: 25647151]
  10. Mol Biol Evol. 2016 Jul;33(7):1870-4 [PMID: 27004904]
  11. Bioinformatics. 2003 Aug 12;19(12):1572-4 [PMID: 12912839]
  12. Zootaxa. 2018 Mar 21;4399(2):197-206 [PMID: 29690304]

Word Cloud

Created with Highcharts 10.0.0nspspeciesμmnewmolecularbasedfemalelongwell-developedITSgenuscollectedcoconutfiberusedgrowingmediastaghornfernsinterceptedimportquarantinedescribedillustratedhereinmorphologicalstudiescharacterizedbodylength728612-846641511-720malerespectivelydelicatestylet8074-84sixlineslateralfieldmedianbulbesophagusmuscularcrescenticvalvepost-vulvaluterinesac3622-52tailelongate-conoidfinelyroundedterminusresultsphylogeneticanalysessequencesD2D3expansionregion28SrRNAconfirmedcloserelationshipsuccessfullyrearedHoweverreproduceculturingcallusedcarrotdisksalfalfaseedlingsMorphologicalMolecularCharacterizationNovelFungal-feedingStemNematodeTylenchida:AnguinidaeInterceptedSamplesDitylenchoidesfungal-feedingphylogenymorphologymorphometricspartialLSUtaxonomy

Similar Articles

Cited By

No available data.