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Environmental microbiology
Mar, 2025;27 (3): e70063.

Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea.

Timothy Klein, Logan H Hodgskiss, Max Dreer, J Colin Murrell, Matthew I Hutchings, Christa Schleper, Laura E Lehtovirta-Morley
Author Information
  1. Timothy Klein: School of Biological Sciences, University of East Anglia, Norwich, UK.
  2. Logan H Hodgskiss: Department of Functional and Evolutionary Ecology, Archaea Biology and Ecogenomics Unit, University of Vienna, Vienna, Austria.
  3. Max Dreer: Department of Functional and Evolutionary Ecology, Archaea Biology and Ecogenomics Unit, University of Vienna, Vienna, Austria.
  4. J Colin Murrell: School of Environmental Sciences, University of East Anglia, Norwich, UK.
  5. Matthew I Hutchings: Department of Molecular Microbiology, John Innes Centre, Norwich, UK.
  6. Christa Schleper: Department of Functional and Evolutionary Ecology, Archaea Biology and Ecogenomics Unit, University of Vienna, Vienna, Austria.
  7. Laura E Lehtovirta-Morley: School of Biological Sciences, University of East Anglia, Norwich, UK. ORCID
PMID: 40070055 DOI: 10.1111/1462-2920.70063

Abstract

Ammonia-oxidising archaea (AOA) are important microorganisms contributing towards the nitrogen flux in the environment. Unlike archaea from other major phyla, genetic tools are yet to be developed for the AOA, and identification of antibiotic resistance markers for selecting mutants is required for a genetic system. The aim of this study was to test the effects of selected antibiotics (hygromycin B, neomycin, apramycin, puromycin, novobiocin) on pure cultures of three well studied AOA strains, 'Candidatus Nitrosocosmicus franklandianus C13', Nitrososphaera viennensis EN76 and Nitrosopumilus maritimus SCM1. Puromycin, hygromycin B and neomycin inhibited some but not all tested archaeal strains. All strains were resistant to apramycin and inhibited by novobiocin to various degrees. As N. viennensis EN76 was relatively more resistant to the tested antibiotics, a wider range of concentrations and compounds (chloramphenicol, trimethoprim, statins) was tested against this strain. N. viennensis EN76 was inhibited by trimethoprim, but not by chloramphenicol, and growth recovered within days in the presence of simvastatin, suggesting either degradation of, or spontaneous resistance against, this compound. This study highlights the physiological differences between different genera of AOA and has identified new candidate antibiotics for selective enrichment and the development of selectable markers for genetic systems in AOA.

Keywords

ammonia���oxidising archaea antibiotics genetic system inhibition selective enrichment

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Grants

  1. ActionR 101079299/HORIZON EUROPE European Research Council
  2. UNITY 852993/European Research Council
  3. W1257/Austrian Science Fund
  4. NE/S007334/1/Natural Environment Research Council
  5. DH150187/Royal Society

MeSH Term

Anti-Bacterial Agents
Archaea
Ammonia
Oxidation-Reduction
Microbial Sensitivity Tests

Chemicals

Anti-Bacterial Agents
Ammonia
Journal Article
Article has an altmetric score of 8

Word Cloud

Created with Highcharts 10.0.0AOAgeneticantibioticsarchaeastrainsviennensisEN76inhibitedtestedresistancemarkerssystemstudyhygromycinBneomycinapramycinnovobiocinresistantNchloramphenicoltrimethoprimselectiveenrichmentAmmonia-oxidisingimportantmicroorganismscontributingtowardsnitrogenfluxenvironmentUnlikemajorphylatoolsyetdevelopedidentificationantibioticselectingmutantsrequiredaimtesteffectsselectedpuromycinpureculturesthreewellstudied'CandidatusNitrosocosmicusfranklandianusC13'NitrososphaeraNitrosopumilusmaritimusSCM1PuromycinarchaealvariousdegreesrelativelywiderrangeconcentrationscompoundsstatinsstraingrowthrecoveredwithindayspresencesimvastatinsuggestingeitherdegradationspontaneouscompoundhighlightsphysiologicaldifferencesdifferentgeneraidentifiednewcandidatedevelopmentselectablesystemsDistinctPatternsAntibioticSensitivitiesAmmonia-OxidisingArchaeaammonia���oxidisinginhibition

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