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Journal of the experimental analysis of behavior
Mar, 2025;123 (2): 337-354.

The rodent electronic nicotine delivery system: Apparatus for voluntary nose-only e-cigarette aerosol inhalation.

Amy L Odum, Mariah E Willis-Moore, Kiernan T Callister, Jeremy M Haynes, Charles C J Frye, Lucy N Scribner, David N Legaspi, Daniel Santos Da Silva, Aaron L Olsen, Tadd T Truscott, Preston T Alden, Rick A Bevins, Adam M Leventhal, Stephen T Lee, Brenna Gomer, Abby D Benninghoff
Author Information
  1. Amy L Odum: Utah State University, Logan, UT, USA. ORCID
  2. Mariah E Willis-Moore: Utah State University, Logan, UT, USA. ORCID
  3. Kiernan T Callister: Utah State University, Logan, UT, USA. ORCID
  4. Jeremy M Haynes: Temple University, Philadelphia, PA, USA.
  5. Charles C J Frye: Cameron University, Lawton, OK, USA.
  6. Lucy N Scribner: Utah State University, Logan, UT, USA.
  7. David N Legaspi: Utah State University, Logan, UT, USA.
  8. Daniel Santos Da Silva: Utah State University, Logan, UT, USA. ORCID
  9. Aaron L Olsen: Utah State University, Logan, UT, USA.
  10. Tadd T Truscott: King Abdullah University of Science and Technology, Thuwal, Makkah Province, Saudi Arabia. ORCID
  11. Preston T Alden: Utah State University, Logan, UT, USA.
  12. Rick A Bevins: University of Nebraska-Lincoln, Lincoln, NE, USA. ORCID
  13. Adam M Leventhal: University of Southern California, Los Angeles, CA, USA.
  14. Stephen T Lee: Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, UT, USA.
  15. Brenna Gomer: University of Utah, Salt Lake City, UT, USA.
  16. Abby D Benninghoff: Utah State University, Logan, UT, USA.
PMID: 40085141 DOI: 10.1002/jeab.70005

Abstract

Tobacco use is the leading cause of death globally and in the United States. After decades of decline, driven by decreases in combusted tobacco use, nicotine product use has increased due to electronic nicotine delivery systems, also known as e-cigarettes or vapes. Preclinical models of nicotine self-administration can serve as important lodestars in the search for effective intervention and prevention tactics. Current variants of the preclinical models have substantial limitations, however. Therefore, we created the rodent electronic nicotine delivery system (RENDS), a novel low-cost nonproprietary nose-only preclinical model of nicotine aerosol self-administration. We confirmed that RENDS sequesters nicotine aerosol in the nose port by measuring fine particulate matter (PM���<2.5 microns) generated by e-cigarettes. We also showed that rats robustly self-administer flavored nicotine aerosol, resulting in high blood levels of cotinine (the major nicotine metabolite) and spontaneous somatic withdrawal symptoms. Thus, we provide validation of the operation and function of the RENDS, opening the door to an open-source preclinical aerosol model of nicotine self-administration that is relatively low in cost. Four existing operant chambers can be retrofitted with the RENDS for less than $325/chamber. All RENDS diagrams and plans for custom-designed components are on Open Science Framework (https://osf.io/x2pqf/?view_only=775b55435b8e428f98e6da384ef7889d).

Keywords

nicotine aerosol nose���only preclinical rats self���administration vaping

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Grants

  1. RD21 DA053818/NIDA NIH HHS
  2. /Utah State University
  3. RD21 DA053818/NIDA NIH HHS

MeSH Term

Animals
Electronic Nicotine Delivery Systems
Rats
Nicotine
Aerosols
Male
Self Administration
Administration, Inhalation
Rats, Sprague-Dawley
Vaping
Cotinine
Substance Withdrawal Syndrome

Chemicals

Nicotine
Aerosols
Cotinine
Journal Article
Article has an altmetric score of 10

Word Cloud

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