An electrochemical DNA biosensor based on denatured vesicle-mediated chain exchange amplification combined with electric field-assistance for nucleic acid detection.
Jinling Cui, Ritong Sun, Yu Liao, Yanling Wang, Chao Jiang, Lei Wang, Chao Shi, Cuiping Ma
Author Information
Jinling Cui: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.
Ritong Sun: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.
Yu Liao: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.
Yanling Wang: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.
Chao Jiang: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.
Lei Wang: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com.
Chao Shi: Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, The Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, P. R. China. ORCID
Cuiping Ma: College of Chemistry and Molecular Engineering, College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China. mcp169@163.com. ORCID
Electrochemical DNA biosensors have been extensively used in food safety, clinical medicine and environmental monitoring due to their high specificity and sensitivity. However, electrochemical DNA biosensors based on nucleic acid hybridization still face challenges in achieving rapid and sensitive detection. In this study, a sensitive and rapid electrochemical DNA biosensor was developed using Strand Exchange Amplification (SEA) technology, with its performance evaluated against the bovine genome as the target. Additionally, gold nanoparticles (AuNPs) were employed to modify the electrode surface, a strategy to enhance both the density of probe modification and the amplification efficiency. Furthermore, the biosensor's sensitivity has been shown to be augmented by the exceptional conductivity of AuNPs. Despite the biosensor's simplicity and sensitivity, the detection time remains a limiting factor. To address this, the incorporation of an electric field within the biosensor framework has been proposed as a strategy to enhance the coupling rate of the nucleic acid amplification and streptavidin-biotin systems. This modification is anticipated to reduce the overall detection time, enabling rapid and precise real-time nucleic acid analysis. The biosensor demonstrated the capability to detect genome DNA as low as 1 fg μL within 65 min, underscoring its significant potential for applications, such as detecting meat adulteration.
