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Feb 26, 2025;17 (3):

In Vitro System for Studying Ilh��us Virus, a Neglected Arbovirus: Ultrastructural Characterization of Cytopathology, Morphology, and Morphogenesis.

Maycon Douglas do Nascimento Garcia, Igor Pinto Silva Da Costa, Marcos Alexandre Nunes da Silva, Vivian Neuza Dos Santos Ferreira, Ana Luisa Teixeira de Almeida, Gabriela Cardoso Caldas, Andressa Santos de Almeida, Ana Maria Bispo de Filippis, Natalia Fintelman-Rodrigues, Aline de Paula Dias da Silva, Marcelo Alves Ferreira, Thiago Moreno L Souza, Alex Pauvolid-Corr��a, Debora Ferreira Barreto-Vieira
Author Information
  1. Maycon Douglas do Nascimento Garcia: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  2. Igor Pinto Silva Da Costa: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
  3. Marcos Alexandre Nunes da Silva: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  4. Vivian Neuza Dos Santos Ferreira: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  5. Ana Luisa Teixeira de Almeida: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  6. Gabriela Cardoso Caldas: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  7. Andressa Santos de Almeida: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  8. Ana Maria Bispo de Filippis: Laborat��rio de Arbov��rus e V��rus Hemorr��gicos, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  9. Natalia Fintelman-Rodrigues: Laborat��rio de Imunofarmacologia, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
  10. Aline de Paula Dias da Silva: Laborat��rio de Imunofarmacologia, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  11. Marcelo Alves Ferreira: Centro de Desenvolvimento Tecnol��gico em Sa��de, National Institute for Science and Technology on Innovation on Diseases of Neglected Populations, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil.
  12. Thiago Moreno L Souza: Laborat��rio de Imunofarmacologia, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  13. Alex Pauvolid-Corr��a: Laborat��rio de Virologia Veterin��ria de Vi��osa, Departamento de Veterin��ria, Universidade Federal de Vi��osa, Vi��osa 36570-900, MG, Brazil. ORCID
  14. Debora Ferreira Barreto-Vieira: Laborat��rio de Morfologia e Morfog��nese Viral, Instituto Oswaldo Cruz, Funda����o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
PMID: 40143249 DOI: 10.3390/v17030320

Abstract

Ilh��us Virus (ILHV) was first detected in 1944 in Ilh��us, state of Bahia, northeast Brazil. During cellular infection, orthoflaviviruses induce cellular changes related both to the replication process, the formation of replication complexes, and to structures resulting from cellular damage. Although more detailed data are available in the literature for other orthoflaviviruses, the relationship between ILHV, the formation of these structures, its replication cycle, and cellular changes remains unknown. One of the main objectives of this study is to characterize the primary ultrastructural changes in green monkey kidney epithelial cell lineage (Vero cell) infected with ILHV, as well as to map its replication cycle, virion structure, and genome. To achieve these objectives, Vero cell monolayers were infected with an MOI of 0.01 and collected at different times post-infection. Cell monolayers were evaluated under bright-field microscopy and transmission electron microscopy. Ultrastructural analyses confirmed that ILHV can induce the formation of double-membrane vesicles, convoluted membranes, and vesicular packets. These structures, like those observed in zika (ZIKV) and dengue (DENV) viruses, form replication complexes that aid ILHV's replication process in cells. Our preliminary results reveal that ILHV infection induces cytopathogenesis like that observed in vitro studies for other arboviruses.

Keywords

Ilh��us virus cytopathology morphogenesis morphology neglected arbovirus ultrastructural studies

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Grants

  1. E-27/2021/269465/Funda����o Carlos Chagas Filho de Amparo �� Pesquisa do Estado do Rio de Janeiro
  2. IOC-023-FIO-18-2-58/FIOTEC

MeSH Term

Animals
Chlorocebus aethiops
Vero Cells
Virus Replication
Microscopy, Electron, Transmission
Arboviruses
Cytopathogenic Effect, Viral
Virion
Genome, Viral
Brazil
Journal Article

Word Cloud

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