Using corn cob powder to induce AFB-degrading enzyme production in Cladosporium uredinicola HSK8 for detoxification purposes.
Mengxiong Xiang, Ernuo Tian, Shuting Wang, Siyao Wang, Runmei Zhang, Jianyi Guo, Ming Liu, Qiang Ding, Jun Cai
Author Information
Mengxiong Xiang: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Ernuo Tian: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Shuting Wang: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Siyao Wang: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Runmei Zhang: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Jianyi Guo: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Ming Liu: Yichang Sanxia Pharmaceutical Co., Ltd, Yichang, 443000, China.
Jun Cai: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China. Electronic address: hgdcaijun@hbut.edu.cn.
Aflatoxin B (AFB) is a highly toxic mycotoxin that contaminates food sources and poses serious health risks. This study examines Cladosporium uredinicola HSK8's ability to degrade AFB. It was demonstrated that the hydrolysis of corn cob powder, following high-temperature treatment, generates derivatives such as furfural and vanillin, which stimulate the production of various AFB-degrading enzymes by C. uredinicola HSK8. The total enzymatic activity reached a peak of 3750 U/mL in fermentation broth with 3 % corn cob powder. Two AFB-degrading enzymes were further isolated, purified and analyzed. The results showed that enzymes can disrupt both the furan and benzene rings, exhibiting strong catalytic activity. The results suggested corn cob powder as a complex carbon source not only promotes the growth of C. uredinicola but also induces the production of AFB-degrading enzymes. Therefore, the fermentation broth of C. uredinicola HSK8 cultured with corn cob powder holds great potential for the detoxification of AFB in food and feed.
