ASL - Plant Editosome Database

Summary

Editing Factor:	ASL
Synonym:	OsASL
Description:	OsASL gene encodes a PPR protein located in the chloroplast, its mutation affects splicing and editing of chloroplast RNAs; OsASL was mainly involved in chlorophyll synthesis pathway
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-DYW
Gene ID & Species:	LOC_Os01g10800 (Oryza sativa)
Edited Gene(s):	ndhA rps14 ndhB rpl2
Editing Type(s):	C-to-U (8)
Publication(s):	[1] A novel PLS-DYW type PPR protein OsASL is essential for chloroplast development in rice., Plant science : an international journal of experimental plant biology, 2024. [PMID=38810885]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os01g10800

Chloroplast

ndhA

473

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ASL	Control	Control	No mutant	Normal	Seedling	NA	Sequencing the RT-PCR product	100.00%	Complete	None	38810885
NA	asl	Knockout	To verify the function of OsASL, a double-target knockout vector was constructed using CRISPR/Cas9 system to knock out the gene; Both targets were close to the 5' end of CDS region; The constructed ve	Knockout	An exhibited albino-lethal phenotype	Seedling	NA	Sequencing the RT-PCR product	0.00%	Unedited	Absent	38810885

Oryza sativa

LOC_Os01g10800

Chloroplast

ndhB

737

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ASL	Control	Control	No mutant	Normal	Seedling	NA	Sequencing the RT-PCR product	74.29%	High	None	38810885
NA	asl	Knockout	To verify the function of OsASL, a double-target knockout vector was constructed using CRISPR/Cas9 system to knock out the gene; Both targets were close to the 5' end of CDS region; The constructed ve	Knockout	An exhibited albino-lethal phenotype	Seedling	NA	Sequencing the RT-PCR product	32.91%	Low	Decreased	38810885

Oryza sativa

LOC_Os01g10800

Chloroplast

rpl2

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ASL	Control	Control	No mutant	Normal	Seedling	NA	Sequencing the RT-PCR product	50.00%	Medium	None	38810885
NA	asl	Knockout	To verify the function of OsASL, a double-target knockout vector was constructed using CRISPR/Cas9 system to knock out the gene; Both targets were close to the 5' end of CDS region; The constructed ve	Knockout	An exhibited albino-lethal phenotype	Seedling	NA	Sequencing the RT-PCR product	82.81%	High	Increased	38810885

Oryza sativa

LOC_Os01g10800

Chloroplast

rps14

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ASL	Control	Control	No mutant	Normal	Seedling	NA	Sequencing the RT-PCR product	80.95%	High	None	38810885
NA	asl	Knockout	To verify the function of OsASL, a double-target knockout vector was constructed using CRISPR/Cas9 system to knock out the gene; Both targets were close to the 5' end of CDS region; The constructed ve	Knockout	An exhibited albino-lethal phenotype	Seedling	NA	Sequencing the RT-PCR product	0.00%	Unedited	Absent	38810885