Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Xuzhou 142 | CLCrV-A | Control | Empty-vector control | No mutant | Normal | NA | NA | RT-PCR and Sanger sequencing | 85.60% | High | None | 40107517 |
| Xuzhou 142 | GhCTEF2-V1 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 51.30% | Medium | Decreased | 40107517 |
| Xuzhou 142 | GhCTEF2-V2 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 55.70% | Medium | Decreased | 40107517 |
| Xuzhou 142 | GhCTEF2-V3 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 67.70% | High | Decreased | 40107517 |
| Xuzhou 142 | CLCrV-A | Control | Empty-vector control | No mutant | Normal | NA | NA | RT-PCR and Sanger sequencing | 76.50% | High | None | 40107517 |
| Xuzhou 142 | GhCTEF2-V1 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 41.70% | Medium | Decreased | 40107517 |
| Xuzhou 142 | GhCTEF2-V2 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 46.90% | Medium | Decreased | 40107517 |
| Xuzhou 142 | GhCTEF2-V3 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 52.30% | Medium | Decreased | 40107517 |
| Xuzhou 142 | CLCrV-A | Control | Empty-vector control | No mutant | Normal | NA | NA | RT-PCR and Sanger sequencing | 84.90% | High | None | 40107517 |
| Xuzhou 142 | GhCTEF2-V1 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 42.70% | Medium | Decreased | 40107517 |
| Xuzhou 142 | GhCTEF2-V2 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 50.80% | Medium | Decreased | 40107517 |
| Xuzhou 142 | GhCTEF2-V3 | Virus-induced gene silencing | Amplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as c | Knockdown | Macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plants | NA | NA | RT-PCR and Sanger sequencing | 56.00% | Medium | Decreased | 40107517 |
