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CTEF2 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: CTEF2
Synonym: GhCTEF2
Description: Photosynthetic efficiency; Thylakoids and grana structures; Transcription levels of PEP-dependent genes; The content of the chloroplast LHCII-T complex protein
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: GhMORF2
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: NA (Gossypium hirsutum)
Edited Gene(s): ndhB    accD    rps18
Editing Type(s): C-to-U (24)
Publication(s): [1] GhCTEF2 encodes a PLS-type PPR protein required for chloroplast development and plastid RNA editing in cotton, Plant Sci, 2025. [doi:10.1016/j.plantsci.2025.112478]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Gossypium hirsutum NA Plastid accD NA NA NA NA Transcript 1403 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Xuzhou 142CLCrV-AControlEmpty-vector controlNo mutantNormalNANART-PCR and Sanger sequencing76.50%HighNone40107517
Xuzhou 142GhCTEF2-V1Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing41.70%MediumDecreased40107517
Xuzhou 142GhCTEF2-V2Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing46.90%MediumDecreased40107517
Xuzhou 142GhCTEF2-V3Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing52.30%MediumDecreased40107517
Gossypium hirsutum NA Plastid accD NA NA NA NA Transcript 1421 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Xuzhou 142CLCrV-AControlEmpty-vector controlNo mutantNormalNANART-PCR and Sanger sequencing84.90%HighNone40107517
Xuzhou 142GhCTEF2-V1Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing42.70%MediumDecreased40107517
Xuzhou 142GhCTEF2-V2Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing50.80%MediumDecreased40107517
Xuzhou 142GhCTEF2-V3Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing56.00%MediumDecreased40107517
Gossypium hirsutum NA Plastid accD NA NA NA NA Transcript 812 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Xuzhou 142CLCrV-AControlEmpty-vector controlNo mutantNormalNANART-PCR and Sanger sequencing85.60%HighNone40107517
Xuzhou 142GhCTEF2-V1Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing51.30%MediumDecreased40107517
Xuzhou 142GhCTEF2-V2Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing55.70%MediumDecreased40107517
Xuzhou 142GhCTEF2-V3Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing67.70%HighDecreased40107517
Gossypium hirsutum NA Plastid ndhB NA NA NA NA Transcript 1255 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Xuzhou 142CLCrV-AControlEmpty-vector controlNo mutantNormalNANART-PCR and Sanger sequencing98.50%HighNone40107517
Xuzhou 142GhCTEF2-V1Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing93.00%HighSimilar40107517
Xuzhou 142GhCTEF2-V2Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing95.20%HighSimilar40107517
Xuzhou 142GhCTEF2-V3Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing71.20%HighDecreased40107517
Gossypium hirsutum NA Plastid ndhB NA NA NA NA Transcript 1481 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Xuzhou 142CLCrV-AControlEmpty-vector controlNo mutantNormalNANART-PCR and Sanger sequencing91.10%HighNone40107517
Xuzhou 142GhCTEF2-V1Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing90.70%HighSimilar40107517
Xuzhou 142GhCTEF2-V2Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing62.30%HighDecreased40107517
Xuzhou 142GhCTEF2-V3Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing63.40%HighDecreased40107517
Gossypium hirsutum NA Plastid rps18 NA NA NA NA Transcript 221 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Xuzhou 142CLCrV-AControlEmpty-vector controlNo mutantNormalNANART-PCR and Sanger sequencing58.00%MediumNone40107517
Xuzhou 142GhCTEF2-V1Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing39.10%LowDecreased40107517
Xuzhou 142GhCTEF2-V2Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing6.10%PoorDecreased40107517
Xuzhou 142GhCTEF2-V3Virus-induced gene silencingAmplifying a GhCTEF2 cDNA fragment of approximately 400 bp; The PCR product was cloned into the pCLCrVA vector to produce a VIGS vector named pCLCrVA-GhCTEF2 and the empty pCLCrVA vector was used as cKnockdownMacular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency; The contents of chlorophyll a, chlorophyll b and total chlorophyll in GhCTEF2-silenced plants were significantly reduced compared with the CLCrV-A plantsNANART-PCR and Sanger sequencing38.30%LowDecreased40107517
Last update: Sep 2026 (version 2.0)