EMP18 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: EMP18
Synonym: Empty Pericarp18
Description: Null mutation of Emp18 arrests embryo and endosperm development at an early stage in maize, resulting in embryo lethality; Mutants are deficient in the cytidine (C)-to-uridine (U) editing at atp6-635 and cox2-449, which converts a Leu to Pro in ATP6 and a Met to Thr in Cox2; The atp6 gene encodes the subunit a of F1 Fo -ATPase; The Leu to Pro alteration disrupts an α-helix of subunit a, resulting in a dramatic reduction in assembly and activity of F1 Fo -ATPase holoenzyme and an accumulation of free F1 -subcomplex
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E1/E2-E+-DYW
Gene ID & Species: GRMZM2G471348 (Zea mays)
Edited Gene(s): atp6    cox2
Editing Type(s): C-to-U (6)
Publication(s): [1] EMP18 functions in mitochondrial atp6 and cox2 transcript editing and is essential to seed development in maize., The New phytologist, 2019. [PMID=30168136]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays GRMZM2G471348 Mitochondrion atp6 635 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WTControlControlControlNormalKernel10-15 DAPqRT-PCR and sequencing100.00%CompleteNone30168136
W22emp18-1Mutator (Mu) insertionA Mu insertion in the E2 domain of the EMP18 proteinHeterozygousEmbryo lethalityKernel10-15 DAPqRT-PCR and sequencing0.00%UneditedAbsent30168136
W22emp18-1Mutator (Mu) insertionA Mu insertion in the E+ domain of the EMP18 proteinHeterozygousEmbryo lethalityKernel10-15 DAPqRT-PCR and sequencing0.00%UneditedAbsent30168136
Zea mays GRMZM2G471348 Mitochondrion cox2 449 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WTControlControlControlNormalKernel10-15 DAPqRT-PCR and sequencing100.00%CompleteNone30168136
W22emp18-2Mutator (Mu) insertionA Mu insertion in the E2 domain of the EMP18 proteinHeterozygousEmbryo lethalityKernel10-15 DAPqRT-PCR and sequencing0.00%UneditedAbsent30168136
W22emp18-2Mutator (Mu) insertionA Mu insertion in the E+ domain of the EMP18 proteinHeterozygousEmbryo lethalityKernel10-15 DAPqRT-PCR and sequencing50.00%MediumDecreased30168136
Last update: Jul 2021 (version 1.0)