EMP18 - Plant Editosome Database - BIG Data Center
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- EMP18
Summary
| Editing Factor: |
EMP18 |
| Synonym: |
NA |
| Description: |
NA |
| Protein Family: |
PPR
|
| Subclass: |
DYW
|
| Construct Structure: |
PLS-E1/E2-E+-DYW
|
| Gene ID & Species: |
GRMZM2G471348 (Zea mays)
|
| Edited Gene(s): |
atp6 cox2
|
| Editing Type(s): |
C-to-U (6)
|
| Publication(s): |
[1] EMP18 Functions in Mitochondrial Atp6 and Cox2 Transcript Editing and Is Essential to Seed Development in Maize, New Phytol, 2018. [PMID=30168136]
|
Editing Details
| Species |
Gene ID |
Organelle |
Edited Gene |
Position |
Region |
Editing Type |
Codon |
Amino Acid |
Molecular Effect |
Experiment Details |
| Zea mays |
GRMZM2G471348 |
Mitochondrion |
atp6
|
635 |
CDS |
C-to-U |
CCG=>CUG |
P=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| W22 | WT | No treatment | No treatment | No treatment | Normal | Kernel | 10-15 DAP | qRT-PCR and sequencing | 100.00% | Complete | None | 30168136 | | W22 | emp18‐1 | Mutator (Mu) insertion | A Mu insertion in the E2 domain of the EMP18 protein | Heterozygous | Embryo lethality | Kernel | 10-15 DAP | qRT-PCR and sequencing | 0.00% | Unedited | Absent | 30168136 | | W22 | emp18‐2 | Mutator (Mu) insertion | A Mu insertion in the E+ domain of the EMP18 protein | Heterozygous | Embryo lethality | Kernel | 10-15 DAP | qRT-PCR and sequencing | 0.00% | Unedited | Absent | 30168136 |
|
| Zea mays |
GRMZM2G471348 |
Mitochondrion |
cox2
|
449 |
CDS |
C-to-U |
ACG=>AUG |
T=>M |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| W22 | WT | No treatment | No treatment | No treatment | Normal | Kernel | 10-15 DAP | qRT-PCR and sequencing | 100.00% | Complete | None | 30168136 | | W22 | emp18‐1 | Mutator (Mu) insertion | A Mu insertion in the E2 domain of the EMP18 protein | Heterozygous | Embryo lethality | Kernel | 10-15 DAP | qRT-PCR and sequencing | 0.00% | Unedited | Absent | 30168136 | | W22 | emp18‐2 | Mutator (Mu) insertion | A Mu insertion in the E+ domain of the EMP18 protein | Heterozygous | Embryo lethality | Kernel | 10-15 DAP | qRT-PCR and sequencing | 50.00% | Medium | Decreased | 30168136 |
|
Last update: Jul 2021
(version 1.0)