EMP80 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM5G851564

Mitochondrion

atp4

118

B73

C-to-U

CGU=>UCU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Ear	12 DAP	Sequencing the amplified products	100.00%	Complete	None	35243635
NA	WT	Control	Control	No mutant	Normal	Ear	8 DAP	Sequencing the amplified products	100.00%	Complete	None	35243635
NA	emp80-1	UniformMu insertion	The emp80 maize mutant alleles in the nearly isogenic W22 background were isolated from the UniformMu transposon population.	Recessive; Heterozygous	The size of mutant kernels is smaller, the pericarp is white wrinkled, and the seed filling is seriously reduced compared to the WT siblings. Embryo and endosperm development are severely inhibited in emp80-1 mutant.	Ear	12 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635
NA	emp80-1	UniformMu insertion	The emp80 maize mutant alleles in the nearly isogenic W22 background were isolated from the UniformMu transposon population.	Recessive; Heterozygous	The size of mutant kernels is smaller, the pericarp is white wrinkled, and the seed filling is seriously reduced compared to the WT siblings. Embryo and endosperm development are severely inhibited in emp80-1 mutant.	Ear	8 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635
NA	emp80-2	UniformMu insertion	NA	Recessive; Heterozygous	Normal	Ear	12 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635
NA	emp80-2	UniformMu insertion	NA	Recessive; Heterozygous	Normal	Ear	8 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635

Zea mays

GRMZM5G851564

Mitochondrion

cox2

482

B73

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Ear	12 DAP	Sequencing the amplified products	100.00%	Complete	None	35243635
NA	emp80-1	UniformMu insertion	The emp80 maize mutant alleles in the nearly isogenic W22 background were isolated from the UniformMu transposon population.	Recessive; Heterozygous	The size of mutant kernels is smaller, the pericarp is white wrinkled, and the seed filling is seriously reduced compared to the WT siblings. Embryo and endosperm development are severely inhibited in emp80-1 mutant.	Ear	12 DAP	Sequencing the amplified products	64.57%	High	Decreased	35243635
NA	emp80-2	UniformMu insertion	NA	Recessive; Heterozygous	Normal	Ear	12 DAP	Sequencing the amplified products	33.54%	Low	Decreased	35243635

Zea mays

GRMZM5G851564

Mitochondrion

nad7

769

B73

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Ear	12 DAP	Sequencing the amplified products	100.00%	Complete	None	35243635
NA	WT	Control	Control	No mutant	Normal	Ear	8 DAP	Sequencing the amplified products	100.00%	Complete	None	35243635
NA	emp80-1	UniformMu insertion	The emp80 maize mutant alleles in the nearly isogenic W22 background were isolated from the UniformMu transposon population.	Recessive; Heterozygous	The size of mutant kernels is smaller, the pericarp is white wrinkled, and the seed filling is seriously reduced compared to the WT siblings. Embryo and endosperm development are severely inhibited in emp80-1 mutant.	Ear	12 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635
NA	emp80-1	UniformMu insertion	The emp80 maize mutant alleles in the nearly isogenic W22 background were isolated from the UniformMu transposon population.	Recessive; Heterozygous	The size of mutant kernels is smaller, the pericarp is white wrinkled, and the seed filling is seriously reduced compared to the WT siblings. Embryo and endosperm development are severely inhibited in emp80-1 mutant.	Ear	8 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635
NA	emp80-2	UniformMu insertion	NA	Recessive; Heterozygous	Normal	Ear	12 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635
NA	emp80-2	UniformMu insertion	NA	Recessive; Heterozygous	Normal	Ear	8 DAP	Sequencing the amplified products	0.00%	Unedited	Decreased	35243635

Editing Factor:	EMP80
Synonym:	Empty Pericarp 80
Description:	An absence of EMP80 abolishes the C-to-U RNA editing at nad7-769 and atp4-118 sites, resulting in a cysteine-to-arginine (Cys-Arg) change in Nad7 and Atp4 in the emp80 mutant; The amino acid change consequently reduces the assembly of complexes I and V, leading to an accumulation of the F1 subcomplex of complex V
Protein Family:	PPR
Subclass:	E+
Physical Interaction:	ZmDYW2
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	GRMZM5G851564 (Zea mays)
Edited Gene(s):	nad7 atp4 cox2
Editing Type(s):	C-to-U (15)
Publication(s):	[1] EMP80 mediates the C-to-U editing of nad7 and atp4 and interacts with ZmDYW2 in maize mitochondria., The New phytologist, 2022. [PMID=35243635]

Summary

Editing Details