Plant Editosome Database
a curated database of RNA editosome in plants

MEF11 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: MEF11
Synonym: LOI1; LOVASTATIN INSENSITIVE 1; MITOCHONDRIAL RNA EDITING FACTOR 11
Description: Encoding a pentatricopeptide (PPR) protein that binds single-stranded RNA, the N-terminal portion of the protein can localize to the mitochondria; Mutations in this gene make plants less sensitive to inhibitors of the MEP and MVA pathways of isoprenoid biosynthesis and increase the activity of HMG CoA reductase
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW, PLS-E like-DYW
Gene ID & Species: AT4G14850 (Arabidopsis thaliana)
Edited Gene(s): cox3    nad4    ccmFn2    ccmC    matR
Editing Type(s): C-to-U (55)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The ABA-deficiency suppressor locus HAS2 encodes the PPR protein LOI1/MEF11 involved in mitochondrial RNA editing., Molecular plant, 2015. [PMID=25708384]
[4] A truncated MEF11 protein shows site-specific effects on mitochondrial RNA editing., Plant signaling & behavior, 2010. [PMID=20436293]
[5] The PPR protein encoded by the LOVASTATIN INSENSITIVE 1 gene is involved in RNA editing at three sites in mitochondria of Arabidopsis thaliana., The Plant journal : for cell and molecular biology, 2010. [PMID=19919573]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G14850 Mitochondrion ccmC 568 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq96.47%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract95.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighSimilar28761003
Arabidopsis thaliana AT4G14850 Mitochondrion ccmFn2 344 CDS C-to-U CCA=>CUA
NA=>NA		 P=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAmef11-1Point MutationThe single amino acid is exchanged in the second PPR repeatNANANANADirect Sequencing of PCR Products0.00%UneditedAbsent20436293
Salk_131734mef11-2T-DNA InsertionT-DNA is inserted in the DYW-domainProcessing of the transcripts does not seem to be affectedNANANADirect Sequencing of PCR Products60.00%HighDecreased20436293
Colmef11-1EMS MutantThe EMS mutant causes a single amino acid alteration of Leu to Pheat residue 48 in the second PPR repeathomozygousThe mef11-1 mutant shows slightly increased tolerance against lovastatin. In the presence of lovastatin, root elongation was inhibited more profoundly in the wild-type plants than in the mutant plants.LeafNADirect Sequencing of PCR Products0.00%UneditedAbsent19919573
SALK_131734mef11-2T-DNA InsertionhomozygousNANormal growth under standard greenhouse conditionsLeafNADirect Sequencing of PCR Products60.00%HighDecreased19919573
ColMEF11ComplementationThe wild-type Col MEF11 gene is cloned under the control of the CaMV 35S promoter, and the plasmid DNA was transfected into mef11-1 mutant protoplastsNANALeafNADirect Sequencing of PCR ProductsNALowDecreased19919573
Colmef11-3The mutant sequences selected mef11-3 were transfected into mef11-1 protoplastsGene variant mef11-3 contains a single mutation in the fourth PPR repeat, which changes amino acid 125 from Val to ThrNANALeafNADirect Sequencing of PCR ProductsNAMediumDecreased19919573
Colmef11-4The mutant sequences selecte mef11-4 were transfected into mef11-1 protoplastsThe mef11-4 gene variant with single nucleotide variations in PPR repeats 8 and 12 and in the E-like domainNANALeafNADirect Sequencing of PCR ProductsNAMediumDecreased19919573
NAmef11-2T-DNA InsertionT-DNA is located in the DYW domainNAExhibited a wild-type phenotypeSeedlingNADirect Sequencing of PCR ProductsNAMediumDecreased25708384
SALK_061056mef11-5T-DNA InsertionT-DNA is inserted in the first exonNAHot leaf phenotype similar to mef11-6SeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
Col-0mef11-6γ-Irradiation Induced MutantThree consecutive mutations (a G to C substitution, a 4-nucleotide deletion, and a 2-nucleotide insertion), which introduced a premature stop codon in the coding sequenceNAmef11 plants are more resistant to progressive water stress and seed germination is more sensitive to paclobutrazolSeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
NAWTControlControlControlNormalSeedlingNADirect Sequencing of PCR ProductsNAMediumNone25708384
Col-0WTControlControlControlNANANARNA-seq93.41%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract98.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighSimilar28761003
Arabidopsis thaliana AT4G14850 Mitochondrion cox3 422 CDS C-to-U CCU=>CUU
NA=>NA		 P=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAmef11-1Point MutationThe single amino acid is exchanged in the second PPR repeatNANANANADirect Sequencing of PCR Products0.00%UneditedAbsent20436293
Salk_131734mef11-2T-DNA InsertionT-DNA is inserted in the DYW-domainProcessing of the transcripts does not seem to be affectedNANANADirect Sequencing of PCR Products0.00%UneditedAbsent20436293
Colmef11-1EMS MutantThe EMS mutant causes a single amino acid alteration of Leu to Pheat residue 48 in the second PPR repeathomozygousThe mef11-1 mutant shows slightly increased tolerance against lovastatin. In the presence of lovastatin, root elongation was inhibited more profoundly in the wild-type plants than in the mutant plants.LeafNAA multiplexed SNaPshot approach0.00%UneditedAbsent19919573
SALK_131734mef11-2T-DNA InsertionhomozygousNANormal growth under standard greenhouse conditionsLeafNAA multiplexed SNaPshot approach0.00%UneditedAbsent19919573
ColWTControlControlControlNormalLeafNAA multiplexed SNaPshot approach100.00%CompleteNone19919573
ColMEF11ComplementationThe wild-type Col MEF11 gene is cloned under the control of the CaMV 35S promoter, and the plasmid DNA was transfected into mef11-1 mutant protoplastsNANALeafNADirect Sequencing of PCR Products50.00%MediumDecreased19919573
Colmef11-3The mutant sequences selected mef11-3 were transfected into mef11-1 protoplastsGene variant mef11-3 contains a single mutation in the fourth PPR repeat, which changes amino acid 125 from Val to ThrNANALeafNADirect Sequencing of PCR Products50.00%MediumDecreased19919573
Colmef11-4The mutant sequences selecte mef11-4 were transfected into mef11-1 protoplastsThe mef11-4 gene variant with single nucleotide variations in PPR repeats 8 and 12 and in the E-like domainNANALeafNADirect Sequencing of PCR Products50.00%MediumDecreased19919573
NAmef11-2T-DNA InsertionT-DNA is located in the DYW domainNAExhibited a wild-type phenotypeSeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
SALK_061056mef11-5T-DNA InsertionT-DNA is inserted in the first exonNAHot leaf phenotype similar to mef11-6SeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
Col-0mef11-6γ-Irradiation Induced MutantThree consecutive mutations (a G to C substitution, a 4-nucleotide deletion, and a 2-nucleotide insertion), which introduced a premature stop codon in the coding sequenceNAmef11 plants are more resistant to progressive water stress and seed germination is more sensitive to paclobutrazolSeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
NAWTControlControlControlNormalSeedlingNADirect Sequencing of PCR Products100.00%CompleteNone25708384
Col-0WTControlControlControlNANANARNA-seq97.85%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT4G14850 Mitochondrion matR 1730 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq91.62%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract87.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract74.00%HighDecreased28761003
Arabidopsis thaliana AT4G14850 Mitochondrion nad4 124 CDS C-to-U CUG=>UUG
NA=>NA		 L=>L
NA=>NA		 Synonymous
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAmef11-1Point MutationThe single amino acid is exchanged in the second PPR repeatNANANANADirect Sequencing of PCR Products0.00%UneditedAbsent20436293
Salk_131734mef11-2T-DNA InsertionT-DNA is inserted in the DYW-domainProcessing of the transcripts does not seem to be affectedNANANADirect Sequencing of PCR Products0.00%UneditedAbsent20436293
Colmef11-1EMS MutantThe EMS mutant causes a single amino acid alteration of Leu to Pheat residue 48 in the second PPR repeathomozygousThe mef11-1 mutant shows slightly increased tolerance against lovastatin. In the presence of lovastatin, root elongation was inhibited more profoundly in the wild-type plants than in the mutant plants.LeafNAA multiplexed SNaPshot approach0.00%UneditedAbsent19919573
SALK_131734mef11-2T-DNA InsertionhomozygousNANormal growth under standard greenhouse conditionsLeafNAA multiplexed SNaPshot approachNAPoorDecreased19919573
ColWTControlControlControlNormalLeafNAA multiplexed SNaPshot approach100.00%CompleteNone19919573
ColMEF11ComplementationThe wild-type Col MEF11 gene is cloned under the control of the CaMV 35S promoter, and the plasmid DNA was transfected into mef11-1 mutant protoplastsNANALeafNADirect Sequencing of PCR Products50.00%MediumDecreased19919573
Colmef11-3The mutant sequences selected mef11-3 were transfected into mef11-1 protoplastsGene variant mef11-3 contains a single mutation in the fourth PPR repeat, which changes amino acid 125 from Val to ThrNANALeafNADirect Sequencing of PCR Products50.00%MediumDecreased19919573
Colmef11-4The mutant sequences selecte mef11-4 were transfected into mef11-1 protoplastsThe mef11-4 gene variant with single nucleotide variations in PPR repeats 8 and 12 and in the E-like domainNANALeafNADirect Sequencing of PCR Products50.00%MediumDecreased19919573
NAmef11-2T-DNA InsertionT-DNA is located in the DYW domainNAExhibited a wild-type phenotypeSeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
SALK_061056mef11-5T-DNA InsertionT-DNA is inserted in the first exonNAHot leaf phenotype similar to mef11-6SeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
Col-0mef11-6γ-Irradiation Induced MutantThree consecutive mutations (a G to C substitution, a 4-nucleotide deletion, and a 2-nucleotide insertion), which introduced a premature stop codon in the coding sequenceNAmef11 plants are more resistant to progressive water stress and seed germination is more sensitive to paclobutrazolSeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent25708384
NAWTControlControlControlNormalSeedlingNADirect Sequencing of PCR ProductsNALowNone25708384
Col-0WTControlControlControlNANANARNA-seq98.94%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)