MEF11 - Plant Editosome Database - CNCB-NGDC

MEF11 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	MEF11
Synonym:	LOI1; LOVASTATIN INSENSITIVE 1; MITOCHONDRIAL RNA EDITING FACTOR 11
Description:	In the mef11-2 mutant a T-DNA insert alters the C-terminal part of the DYW domain; This insertion blocks RNA editing at sites cox 3-422 and site nad4-124 completely, but only partially at ccb203-344; The enzymatic activity or its recruitment is not compromised by the alteration of the C-terminus of MEF11; MEF11 is involved in RNA editing of the three sites cox3-422, nad4-124 and ccb203-344 in Arabidopsis thaliana; The mef11-1 mutant described here shows similar tolerance to lovastatin; Identification of the function of the MEF11 protein in site-specific mitochondrial RNA editing suggests indirect effects of retrograde signalling from mitochondria to the cytoplasm to evoke alteration of the mevalonate pathway; Like pp2c, mef11 plants were more resistant to progressive water stress and seed germination was more sensitive to paclobutrazol (a gibberellin biosynthesis inhibitor) as well as mannitol and NaCl, compared with the wild-type plants; Phenotypic alterations in mef11 were associated with the lack of editing of transcripts for the mitochondrial cytochrome c maturation FN2 (ccmFN2) gene, which encodes a cytochrome c-heme lyase subunit involved in cytochrome c biogenesis; Although the abundance of electron transfer chain complexes was not affected, their dysfunction could be deduced from increased respiration and altered production of hydrogen peroxide and nitric oxide in mef11 seeds
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT4G14850 (Arabidopsis thaliana)
Edited Gene(s):	cox3 nad4 ccmFn2 matR ccmC
Editing Type(s):	C-to-U (50)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The ABA-Deficiency Suppressor Locus HAS2 Encodes the PPR Protein LOI1/MEF11 Involved in Mitochondrial RNA Editing, Molecular Plant, 2015. [doi:10.1016/j.molp.2014.12.005] [3] A truncated MEF11 protein shows site-specific effects on mitochondrial RNA editing, Plant Signaling & Behavior, 2010. [doi:10.4161/psb.11095] [4] The PPR protein encoded by the LOVASTATIN INSENSITIVE 1 gene is involved in RNA editing at three sites in mitochondria of Arabidopsis thaliana, The Plant Journal, 2010. [doi:10.1111/j.1365-313X.2009.04076.x]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G14850

Mitochondrion

568

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	Similar	28761003

Arabidopsis thaliana

AT4G14850

Mitochondrion

344

CDS

NA

NA

NA

NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	mef11-1	Point Mutation	The single amino acid is exchanged in the second PPR repeat	NA	NA	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20436293
Salk_131734	mef11-2	T-DNA Insertion	T-DNA is inserted in the DYW-domain	NA	NA	NA	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	20436293
Col	mef11-1	EMS Mutant	The EMS mutant causes a single amino acid alteration of Leu to Pheat residue 48 in the second PPR repeat	homozygous	The mef11-1 mutant shows slightly increased tolerance against lovastatin. In the presence of lovastatin, root elongation was inhibited more profoundly in the wild-type plants than in the mutant plants.	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19919573
SALK_131734	mef11-2	T-DNA Insertion	homozygous	NA	Normal growth under standard greenhouse conditions	Leaf	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	19919573
Col	MEF11	Complementation	The wild-type Col MEF11 gene is cloned under the control of the CaMV 35S promoter, and the plasmid DNA was transfected into mef11-1 mutant protoplasts	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	NA	Low	Decreased	19919573
Col	mef11-3	The mutant sequences selected mef11-3 were transfected into mef11-1 protoplasts	Gene variant mef11-3 contains a single mutation in the fourth PPR repeat, which changes amino acid 125 from Val to Thr	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	19919573
Col	mef11-4	The mutant sequences selecte mef11-4 were transfected into mef11-1 protoplasts	The mef11-4 gene variant with single nucleotide variations in PPR repeats 8 and 12 and in the E-like domain	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	19919573
NA	mef11-2	T-DNA Insertion	T-DNA is located in the DYW domain	NA	Exhibited a wild-type phenotype	Seedling	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	25708384
SALK_061056	mef11-5	T-DNA Insertion	T-DNA is inserted in the first exon	NA	Hot leaf phenotype similar to mef11-6	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
Col-0	mef11-6	γ-Irradiation Induced Mutant	Three consecutive mutations (a G to C substitution, a 4-nucleotide deletion, and a 2-nucleotide insertion), which introduced a premature stop codon in the coding sequence	NA	mef11 plants are more resistant to progressive water stress and seed germination is more sensitive to paclobutrazol	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
NA	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct Sequencing of PCR Products	NA	Medium	None	25708384
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	98.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	Similar	28761003

Arabidopsis thaliana

AT4G14850

Mitochondrion

422

CDS

NA

NA

NA

NA

C-to-U

CCU=>CUU
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	mef11-1	Point Mutation	The single amino acid is exchanged in the second PPR repeat	NA	NA	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20436293
Salk_131734	mef11-2	T-DNA Insertion	T-DNA is inserted in the DYW-domain	NA	NA	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20436293
Col	mef11-1	EMS Mutant	The EMS mutant causes a single amino acid alteration of Leu to Pheat residue 48 in the second PPR repeat	homozygous	The mef11-1 mutant shows slightly increased tolerance against lovastatin. In the presence of lovastatin, root elongation was inhibited more profoundly in the wild-type plants than in the mutant plants.	Leaf	NA	A multiplexed SNaPshot approach	0.00%	Unedited	Absent	19919573
SALK_131734	mef11-2	T-DNA Insertion	homozygous	NA	Normal growth under standard greenhouse conditions	Leaf	NA	A multiplexed SNaPshot approach	0.00%	Unedited	Absent	19919573
Col	WT	Control	Control	No mutant	Normal	Leaf	NA	A multiplexed SNaPshot approach	100.00%	Complete	None	19919573
Col	MEF11	Complementation	The wild-type Col MEF11 gene is cloned under the control of the CaMV 35S promoter, and the plasmid DNA was transfected into mef11-1 mutant protoplasts	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	19919573
Col	mef11-3	The mutant sequences selected mef11-3 were transfected into mef11-1 protoplasts	Gene variant mef11-3 contains a single mutation in the fourth PPR repeat, which changes amino acid 125 from Val to Thr	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	19919573
Col	mef11-4	The mutant sequences selecte mef11-4 were transfected into mef11-1 protoplasts	The mef11-4 gene variant with single nucleotide variations in PPR repeats 8 and 12 and in the E-like domain	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	19919573
NA	mef11-2	T-DNA Insertion	T-DNA is located in the DYW domain	NA	Exhibited a wild-type phenotype	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
SALK_061056	mef11-5	T-DNA Insertion	T-DNA is inserted in the first exon	NA	Hot leaf phenotype similar to mef11-6	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
Col-0	mef11-6	γ-Irradiation Induced Mutant	Three consecutive mutations (a G to C substitution, a 4-nucleotide deletion, and a 2-nucleotide insertion), which introduced a premature stop codon in the coding sequence	NA	mef11 plants are more resistant to progressive water stress and seed germination is more sensitive to paclobutrazol	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
NA	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	25708384
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT4G14850

Mitochondrion

1730

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	87.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	96.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	74.00%	High	Decreased	28761003

Arabidopsis thaliana

AT4G14850

Mitochondrion

124

CDS

NA

NA

NA

NA

C-to-U

CUG=>UUG
NA=>NA

L=>L
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	mef11-1	Point Mutation	The single amino acid is exchanged in the second PPR repeat	NA	NA	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20436293
Salk_131734	mef11-2	T-DNA Insertion	T-DNA is inserted in the DYW-domain	NA	NA	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20436293
Col	mef11-1	EMS Mutant	The EMS mutant causes a single amino acid alteration of Leu to Pheat residue 48 in the second PPR repeat	homozygous	The mef11-1 mutant shows slightly increased tolerance against lovastatin. In the presence of lovastatin, root elongation was inhibited more profoundly in the wild-type plants than in the mutant plants.	Leaf	NA	A multiplexed SNaPshot approach	0.00%	Unedited	Absent	19919573
SALK_131734	mef11-2	T-DNA Insertion	homozygous	NA	Normal growth under standard greenhouse conditions	Leaf	NA	A multiplexed SNaPshot approach	NA	Poor	Decreased	19919573
Col	WT	Control	Control	No mutant	Normal	Leaf	NA	A multiplexed SNaPshot approach	100.00%	Complete	None	19919573
Col	MEF11	Complementation	The wild-type Col MEF11 gene is cloned under the control of the CaMV 35S promoter, and the plasmid DNA was transfected into mef11-1 mutant protoplasts	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	19919573
Col	mef11-3	The mutant sequences selected mef11-3 were transfected into mef11-1 protoplasts	Gene variant mef11-3 contains a single mutation in the fourth PPR repeat, which changes amino acid 125 from Val to Thr	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	19919573
Col	mef11-4	The mutant sequences selecte mef11-4 were transfected into mef11-1 protoplasts	The mef11-4 gene variant with single nucleotide variations in PPR repeats 8 and 12 and in the E-like domain	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	19919573
NA	mef11-2	T-DNA Insertion	T-DNA is located in the DYW domain	NA	Exhibited a wild-type phenotype	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
SALK_061056	mef11-5	T-DNA Insertion	T-DNA is inserted in the first exon	NA	Hot leaf phenotype similar to mef11-6	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
Col-0	mef11-6	γ-Irradiation Induced Mutant	Three consecutive mutations (a G to C substitution, a 4-nucleotide deletion, and a 2-nucleotide insertion), which introduced a premature stop codon in the coding sequence	NA	mef11 plants are more resistant to progressive water stress and seed germination is more sensitive to paclobutrazol	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25708384
NA	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct Sequencing of PCR Products	NA	Low	None	25708384
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Last update: Sep 2026 (version 2.0)