ORRM5 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G13850

Mitochondrion

atp1

1425

CDS

TAIR (v10)

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	62.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	61.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	30.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

atp8

389

CDS

TAIR (v10)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	52.00%	Medium	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	20.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

128

CDS

TAIR (v10)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	64.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	60.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	86.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

137

CDS

TAIR (v10)

C-to-U

UCC/UCC=>UUC/UUU

S=>F/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	73.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	67.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	91.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

148

CDS

TAIR (v10)

C-to-U

CCG/CCG=>UCG/UUG

P=>S/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	73.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	68.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

149

CDS

TAIR (v10)

C-to-U

CCG/CCG=>CUG/UUG

P=>L/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	74.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	69.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

154

CDS

TAIR (v10)

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	70.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	91.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

159

CDS

TAIR (v10)

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	70.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

160

CDS

TAIR (v10)

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	70.00%	High	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

406

CDS

TAIR (v10)

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	62.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	57.00%	Medium	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	23.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

576

CDS

TAIR (v10)

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	74.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	71.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	36.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmFn2

320

CDS

TAIR (v10)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	78.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad3

CDS

TAIR (v10)

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	97.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	95.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	67.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad3

CDS

TAIR (v10)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	95.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	92.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	56.00%	Medium	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad4

1131

CDS

TAIR (v10)

C-to-U

CUC/CUC=>CUU/UUU

L=>L/F

Synonymous/Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	56.00%	Medium	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	52.00%	Medium	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	17.00%	Poor	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad5

Intron

TAIR (v10)

Genomic position

141966

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	79.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	34.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad7

789

CDS

TAIR (v10)

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	79.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	69.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	20.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

rps3

1344

CDS

TAIR (v10)

C-to-U

AGC=>AGU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	83.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	80.00%	High	Increased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	52.00%	Medium	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

rps3

887

CDS

TAIR (v10)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	56.00%	Medium	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	82.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

rps4

175

CDS

TAIR (v10)

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	55.00%	Medium	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	52.00%	Medium	Decreased	28549172
SALK_135802C	WT	Control	Wild-type siblings of ORRM5-3	No mutant	Normal	NA	NA	STS-PCRseq	77.00%	High	None	28549172

Editing Factor:	ORRM5
Synonym:	Organelle RRM Protein 5; ATGRP2; GLYCINE RICH PROTEIN 2; GLYCINE-RICH RNA-BINDING PROTEIN 2; GR-RBP2; GRP2; RBGA5; RNA-BINDING GLYCINE-RICH PROTEIN A5
Description:	Unlike other ORRM editing factors, the absence of ORRM5 in orrm5 mutant plants results in an increase of the editing extent in 14% of the mitochondrial sites surveyed; The orrm5 mutant also exhibits a reduced splicing efficiency of the first nad5 intron and slower growth and delayed flowering time; ORRM5 contains an RNA recognition motif (RRM) and a glycine-rich domain at the C terminus; The RRM provides the editing activity of ORRM5 and is able to complement the splicing but not the morphological defects
Protein Family:	Organelle RNA recognition motif-containing (ORRM)
Subclass:	NA
Physical Interaction:	ORRM2; ORRM3; ORRM4
Construct Structure:	RRM-GR
Gene ID & Species:	AT4G13850 (Arabidopsis thaliana)
Edited Gene(s):	atp8 rps3 ccmFn2 rps4 nad4 ccmB atp1 nad7 nad5 nad3
Editing Type(s):	C-to-U (60)
Publication(s):	[1] ORRM5, an RNA recognition motif-containing protein, has a unique effect on mitochondrial RNA editing, Journal of Experimental Botany, 2017. [doi:10.1093/jxb/erx139]

Summary

Editing Details