ORRM5 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G13850

Mitochondrion

atp1

1425

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	62.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	61.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	30.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

atp8

389

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	52.00%	Medium	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	20.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

128

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	64.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	60.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	86.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

137

CDS

C-to-U

UCC/UCC=>UUC/UUU

S=>F/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	73.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	67.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	91.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

148

CDS

C-to-U

CCG/CCG=>UCG/UUG

P=>S/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	73.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	68.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

149

CDS

C-to-U

CCG/CCG=>CUG/UUG

P=>L/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	74.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	69.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

154

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	70.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	91.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

159

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	70.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

160

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	70.00%	High	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	90.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

406

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	62.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	57.00%	Medium	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	23.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmB

576

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	74.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	71.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	36.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

ccmFN2

320

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	78.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad3

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	95.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	92.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	56.00%	Medium	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad3

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	97.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	95.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	67.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad4

1131

CDS

C-to-U

CUC/CUC=>CUU/UUU

L=>L/F

Synonymous/Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	56.00%	Medium	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	52.00%	Medium	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	17.00%	Poor	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad5

141966

Intronic

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	79.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	75.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	34.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

nad7

789

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	79.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	69.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	20.00%	Low	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

rps3

887

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	54.00%	Medium	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	56.00%	Medium	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	82.00%	High	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

rps3

1344

CDS

C-to-U

AGC=>AGU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	83.00%	High	Increased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	80.00%	High	Increased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	52.00%	Medium	None	28549172

Arabidopsis thaliana

AT4G13850

Mitochondrion

rps4

175

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_059714C	orrm5-2	T-DNA Insertion	T-DNA is inserted in the second exon	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	55.00%	Medium	Decreased	28549172
SALK_135802C	orrm5-3	T-DNA Insertion	T-DNA is inserted in the fourth intron	Knockout; Homozygous	Slower growth and delayed flowering time	NA	NA	STS-PCRseq	52.00%	Medium	Decreased	28549172
SALK_135802C	WT	No treatment	Wild-type siblings of ORRM5-3	No treatment	No treatment	NA	NA	STS-PCRseq	77.00%	High	None	28549172

Editing Factor:	ORRM5
Synonym:	ATGRP2, GLYCINE RICH PROTEIN 2, GLYCINE-RICH RNA-BINDING PROTEIN 2, GR-RBP2, GRP2, RBGA5, RNA-BINDING GLYCINE-RICH PROTEIN A5
Description:	Encodes an RNA recognition motif-containing protein which has an inhibitory impact on plant organelle editing.
Protein Family:	ORRM
Subclass:	NA
Construct Structure:	RRM
Gene ID & Species:	AT4G13850 (Arabidopsis thaliana)
Edited Gene(s):	atp8 rps3 ccmFN2 rps4 nad4 ccmB atp1 nad7 nad5 nad3
Editing Type(s):	C-to-U (60)
Publication(s):	[1] ORRM5, an RNA recognition motif-containing protein, has a unique effect on mitochondrial RNA editing, Journal of Experimental Botany, 2017. [PMID=28549172]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details