PPR27 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G411786

Mitochondrion

ccmFN

1357

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	100.00%	Complete	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050

Zea mays

GRMZM2G411786

Mitochondrion

cox2

482

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	83.33%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	57.89%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	38.46%	Low	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

nad2

355

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	100.00%	Complete	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	75.00%	High	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	60.00%	High	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps12

221

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	71.43%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	57.14%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	50.00%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps13

100

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	71.43%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	64.29%	High	Similar	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	53.33%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps13

256

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	72.00%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	54.54%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	50.00%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps13

287

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	72.00%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	54.54%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	45.45%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps3

707

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	No treatment	No treatment	No treatment	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo and endosperm	13 DAP	PCR and direct sequencing	36.67%	Low	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050

Editing Factor:	PPR27
Synonym:	NA
Description:	NA
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E1-E2-E+-DYW
Gene ID & Species:	GRMZM2G411786 (Zea mays)
Edited Gene(s):	ccmFN rps3 rps12 rps13 nad2 cox2
Editing Type(s):	C-to-U (24)
Publication(s):	[1] The DYW-subgroup Pentatricopeptide Repeat Protein PPR27 Functions on Editing of Multiple Mitochondrial Transcripts and Interacts With ZmMORF1 in Maize, J Exp Bot, 2020. [PMID=32531050]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details