PPR27 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G411786

Mitochondrion

ccmFn

1357

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	100.00%	Complete	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050

Zea mays

GRMZM2G411786

Mitochondrion

cox2

482

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	83.33%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	57.89%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	38.46%	Low	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

nad2

355

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	100.00%	Complete	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	75.00%	High	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	60.00%	High	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps12

221

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	71.43%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	57.14%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	50.00%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps13

100

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	71.43%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	64.29%	High	Similar	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	53.33%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps13

256

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	72.00%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	54.54%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	50.00%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps13

287

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	72.00%	High	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	54.54%	Medium	Decreased	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	45.45%	Medium	Decreased	32531050

Zea mays

GRMZM2G411786

Mitochondrion

rps3

707

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	Control	From 9 DAP to 15 DAP, WT embryos reached the coleoptilar stage, developing primary scutellum, coleoptile and shoot and root primordia.	Immature embryo; Endosperm	13 DAP	PCR and direct sequencing	36.67%	Low	None	32531050
W22	ppr27-1	Mu insertion	The Mu insertion is located at 1110 bp downstream from the ATG in ppr27-1.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype. From 9 DAP to 15 DAP, the ppr27-1 embryos remained at the transition stage.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050
W22	ppr27-2	Mu insertion	The Mu insertion is located at 1054 bp downstream from the ATG in ppr27-2.	Homozygous	Homozygous kernels of both mutants are small and white with an empty pericarp (emp) phenotype.	Kernel	13 DAP	PCR and direct sequencing	0.00%	Unedited	Absent	32531050

Editing Factor:	PPR27
Synonym:	NA
Description:	The ppr27 mutant is completely deficient in C-to-U editing at the ccmFN-1357 and rps3-707 sites, and editing at six other sites is substantially reduced; The lack of editing at ccmFN-1357 causes a deficiency of CcmFN protein; As CcmFN functions in the maturation pathway of cytochrome proteins that are subunits of mitochondrial complex III, its deficiency results in an absence of cytochrome c1 and cytochrome c proteins; Consequently, the assembly of mitochondrial complex III and super-complex I+III2 is decreased, which impairs the electron transport chain and respiration, leading to arrests in embryogenesis and endosperm development in ppr27
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	MORF1
Construct Structure:	PLS-E1-E2-E+-DYW
Gene ID & Species:	GRMZM2G411786 (Zea mays)
Edited Gene(s):	ccmFn rps3 rps12 rps13 nad2 cox2
Editing Type(s):	C-to-U (24)
Publication(s):	[1] The DYW-subgroup pentatricopeptide repeat protein PPR27 interacts with ZmMORF1 to facilitate mitochondrial RNA editing and seed development in maize., Journal of experimental botany, 2020. [PMID=32531050]

Summary

Editing Details