PPR_77 - Plant Editosome Database

Editing Factor:	PPR_77
Synonym:	Phypa_191892
Description:	Disruption of each gene by homologous recombination revealed that PpPPR_56 was involved in RNA editing at the nad3 and nad4 sites, PpPPR_77 at the cox2 and cox3 sites, and PpPPR_91 at the nad5-2 site in the mitochondrial transcripts; The nucleotide sequences surrounding the two editing sites targeted by a single PPR-DYW protein share 42 to 56% of their identities
Protein Family:	Pentatricopeptide repeat (PPR)
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E/E+-DYW
Gene ID & Species:	PP00169G00580 (Physcomitrium patens)
Edited Gene(s):	cox2 cox3
Editing Type(s):	C-to-U (6)
Publication(s):	[1] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts, Plant Cell Physiology, 2010. [PMID=20837503]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Physcomitrium patens

PP00169G00580

Mitochondrion

370

CDS

P. patens subsp. patens genome ver. 1.1

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	△77-16	The Nptii Gene Cassette	Restriction sites - Xhoi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	△77-7	The Nptii Gene Cassette	Restriction sites - Xhoi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	WT	Control	Control	No mutant	Normal	Protonema	3 days	Direct Sequencing of PCR Products	100.00%	Complete	None	20837503