PpPPR_77 - Plant Editosome Database

Editing Factor:	PpPPR_77
Synonym:	Phypa_191892
Description:	Encodes a PPR-DYW protein involved in RNA editing.
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E/E+-DYW
Gene ID & Species:	PP00169G00580 (Physcomitrella patens)
Edited Gene(s):	cox2 cox3
Editing Type(s):	C-to-U (6)
Publication(s):	[1] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts, Plant Cell Physiology, 2010. [PMID=20837503]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Physcomitrella patens

PP00169G00580

Mitochondrion

370

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Physcomitrella patens subsp. Patens	Δ77-16	The Nptii Gene Cassette	Restriction sites - Xhoi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
Physcomitrella patens subsp. Patens	Δ77-7	The Nptii Gene Cassette	Restriction sites - Xhoi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
Physcomitrella patens subsp. Patens	WT	No treatment	No treatment	No treatment	No treatment	Protonema	3 days	Direct Sequencing of PCR Products	100.00%	Complete	None	20837503

Physcomitrella patens

PP00169G00580

Mitochondrion

733

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Physcomitrella patens subsp. Patens	Δ77-16	The Nptii Gene Cassette	Restriction sites - Xhoi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
Physcomitrella patens subsp. Patens	Δ77-7	The Nptii Gene Cassette	Restriction sites - Xhoi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
Physcomitrella patens subsp. Patens	WT	No treatment	No treatment	No treatment	No treatment	Protonema	3 days	Direct Sequencing of PCR Products	100.00%	Complete	None	20837503