RAS - Plant Editosome Database

Editing Factor:	RAS
Synonym:	Rice Albino Seedling-Lethal
Description:	RAS affect the RNA editing and RNA splicing of chloroplast transcripts, as well as the expression of chloroplast-related genes; RAS functions may be regulated and assisted by an SRP-dependent pathway
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	P
Physical Interaction:	OsTRX z; Chloroplast signal recognition particle (cpSRP) protein
Construct Structure:	P-SMR
Gene ID & Species:	LOC_Os03g60910 (Oryza sativa)
Edited Gene(s):	rpl2 ndhA rps14
Editing Type(s):	C-to-U (6)
Publication(s):	[1] RAS, a Pentatricopeptide Repeat Protein, Interacts with OsTRX z to Regulate Chloroplast Gene Transcription and RNA Processing, Plants (Basel), 2025. [doi:10.3390/plants14020247]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os03g60910

Chloroplast

cDNA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	WT	Control	Control	No mutant	Normal	Seedling	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39861600
Zhonghua 11	ras	CRISPR/Cas9 genome editing	A 1 bp insertion and 2 bp deletion at the designated target site	Homozygous	An albino seedling-lethal phenotype characterized by abnormal chloroplast structures and a significantly reduced chlorophyll content	Seedling	NA	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	39861600

Oryza sativa

LOC_Os03g60910

Chloroplast

cDNA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	WT	Control	Control	No mutant	Normal	Seedling	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39861600
Zhonghua 11	ras	CRISPR/Cas9 genome editing	A 1 bp insertion and 2 bp deletion at the designated target site	Homozygous	An albino seedling-lethal phenotype characterized by abnormal chloroplast structures and a significantly reduced chlorophyll content	Seedling	NA	RT-PCR products were directly sequenced	72.50%	High	Decreased	39861600

Oryza sativa

LOC_Os03g60910

Chloroplast

cDNA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	WT	Control	Control	No mutant	Normal	Seedling	NA	RT-PCR products were directly sequenced	76.00%	High	None	39861600
Zhonghua 11	ras	CRISPR/Cas9 genome editing	A 1 bp insertion and 2 bp deletion at the designated target site	Homozygous	An albino seedling-lethal phenotype characterized by abnormal chloroplast structures and a significantly reduced chlorophyll content	Seedling	NA	RT-PCR products were directly sequenced	37.00%	Low	Decreased	39861600