Plant Editosome Database
a curated database of RNA editosome in plants

RPOTp - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: RPOTp
Synonym: NA
Description: RPOTp directly interacts with MORF2, MORF8, MORF9, and ORRM1, affecting the dimerization of MORF proteins in chloroplast RNA editosomes.
Protein Family: RNA polymerase
Subclass: NA
Construct Structure: NA
Gene ID & Species: AT2G24120 (Arabidopsis thaliana)
Edited Gene(s): rpoC1    rps12    ycf3    ndhB    ndhD    ndhF    ndhG
Editing Type(s): C-to-U (27)
Publication(s): [1] RNA Polymerase RPOTp is Involved in C-to-U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts., Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. [PMID=39630097]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G24120 Chloroplast ndhB NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing91.00%HighNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing70.00%HighDecreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing100.00%CompleteRestored39630097
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing89.00%HighNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing52.00%MediumDecreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing100.00%CompleteRestored39630097
Arabidopsis thaliana AT2G24120 Chloroplast ndhD NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing65.00%HighNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing13.00%PoorDecreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing72.00%HighRestored39630097
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing100.00%CompleteNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing74.00%HighDecreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing100.00%CompleteRestored39630097
Arabidopsis thaliana AT2G24120 Chloroplast ndhF NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing96.00%HighNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing74.00%HighDecreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing97.00%HighRestored39630097
Arabidopsis thaliana AT2G24120 Chloroplast ndhG NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing93.00%HighNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing67.00%HighDecreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing93.00%HighRestored39630097
Arabidopsis thaliana AT2G24120 Chloroplast rpoC1 NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing23.00%LowNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing74.00%HighIncreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing26.00%LowRestored39630097
Arabidopsis thaliana AT2G24120 Chloroplast rps12 NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing20.00%LowNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing42.00%MediumIncreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing33.00%LowRestored39630097
Arabidopsis thaliana AT2G24120 Chloroplast ycf3 NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedling4‐day‐oldRT‐PCR and Sanger sequencing5.00%PoorNone39630097
SALK_093884sca3-2A T‐DNA insertionA T‐DNA insertion in the third intron of SCA3 geneHomozygousSmaller pale‐yellow cotyledons and seedling leaves, as well as smaller plants throughout their entire life cycle compared to Col‐0 wild type when grown under standard light conditions; The thylakoids were severely disrupted in the sca3‐2 mutant, and large thylakoid lamellas and transparent vacuoles were also observed in some chloroplasts. Some plastids formed rudimentary thylakoids consisting only of grana lamellae and failed to accumulate stromal lamellae, while others were filled with numerous vesicles; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0Seedling4‐day‐oldRT‐PCR and Sanger sequencing30.00%LowIncreased39630097
NAsca3-2/com1ComplementationTo obtain the complementary transgenic plants (sca3‐2/com) of sca3‐2 mutants, the genomic fragment containing 1.5 kb promoter and the SCA3 gene without stop codon was amplified by PCR using Q5® Hot StHomozygousNormal green cotyledons and leaves phenotype; The chlorophyll content in sca3‐2 mutants is significantly lower compared with Col‐0, and in sca3‐2/com1 plants, it restores to the level of the wild typeSeedling4‐day‐oldRT‐PCR and Sanger sequencing5.00%PoorRestored39630097
Last update: Jul 2021 (version 1.0)