TSL1 - Plant Editosome Database - BIG Data Center

TSL1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	TSL1
Synonym:	NA
Description:	NA
Protein Family:	PPR
Subclass:	P
Construct Structure:	NA
Gene ID & Species:	NA (Arabidopsis thaliana)
Edited Gene(s):	accD ndhD petL
Editing Type(s):	C-to-U (28) G-to-A (14)
Publication(s):	[1] Mutation of DELAYED GREENING1 impairs chloroplast RNA editing at elevated ambient temperature in Arabidopsis, J Genet Genomics, 2020. [PMID=32505546]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

NA

Chloroplast

1568

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	WT	Grown at 22℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	64.29%	High	None	32505546
Columbia	tsl1	Grown at 22℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	NA	tsl1, grew as normally as wild-type (WT) plants	Leaf	NA	RT-PCR and sequencing	58.82%	Medium	Similar	32505546
Columbia	Line 3/tsl1	Grown at 22℃	For the complementation of tsl1 mutant, wild-type genomic DNA sequences of AT5G67570 including the upstream promoter sequences were amplified with PCR and inserted into pCAMBIA1300. The construct was	NA	Line 3/tsl1, grew as normally as wild-type (WT) plants	Leaf	NA	RT-PCR and sequencing	66.67%	High	Restored	32505546
Columbia	WT	Grown at 29℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (29℃) for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	66.67%	High	None	32505546
Columbia	tsl1	Grown at 29℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevatedtemperature conditions (29℃) for 7 days.	NA	The newly developed true leaves of tsl1 plants turned uniformly white.	Leaf	NA	RT-PCR and sequencing	26.67%	Low	Decreased	32505546
Columbia	Line 3/tsl1	Grown at 29℃	For the complementation of tsl1 mutant, wild-type genomic DNA sequences of AT5G67570 including the upstream promoter sequences were amplified with PCR and inserted into pCAMBIA1300. The construct was	NA	NA	Leaf	NA	RT-PCR and sequencing	64.29%	High	Restored	32505546
Columbia	WT	Grown at 26℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (26℃) for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	70.00%	High	None	32505546
Columbia	tsl1	Grown at 26℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (26℃) for 7 days. To prevent possible interference of ivory leaves	NA	The newly developed true leaves of tsl1 plants turned pale green. the plastids in the tsl1 mutant plants were small and abnormally shaped, including less developed thylakoid membranes and starch granules compared to that in the WT plants.	Leaf	NA	RT-PCR and sequencing	37.50%	Low	Decreased	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 0 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	68.00%	High	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 0 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	65.22%	High	Similar	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 1.5 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	54.55%	Medium	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 1.5 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	69.23%	High	Increased	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 3 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	54.55%	Medium	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 3 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	34.78%	Low	Decreased	32505546

Arabidopsis thaliana

NA

Chloroplast

2

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	WT	Grown at 22℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	66.67%	High	None	32505546
Columbia	tsl1	Grown at 22℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	NA	tsl1, grew as normally as wild-type (WT) plants	Leaf	NA	RT-PCR and sequencing	68.75%	High	Similar	32505546
Columbia	Line 3/tsl1	Grown at 22℃	For the complementation of tsl1 mutant, wild-type genomic DNA sequences of AT5G67570 including the upstream promoter sequences were amplified with PCR and inserted into pCAMBIA1300. The construct was	NA	Line 3/tsl1, grew as normally as wild-type (WT) plants	Leaf	NA	RT-PCR and sequencing	66.67%	High	Restored	32505546
Columbia	WT	Grown at 29℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (29℃) for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	None	32505546
Columbia	tsl1	Grown at 29℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevatedtemperature conditions (29℃) for 7 days.	NA	The newly developed true leaves of tsl1 plants turned uniformly white.	Leaf	NA	RT-PCR and sequencing	11.76%	Poor	Decreased	32505546
Columbia	Line 3/tsl1	Grown at 29℃	For the complementation of tsl1 mutant, wild-type genomic DNA sequences of AT5G67570 including the upstream promoter sequences were amplified with PCR and inserted into pCAMBIA1300. The construct was	NA	NA	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Restored	32505546
Columbia	WT	Grown at 26℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (26℃) for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	64.29%	High	None	32505546
Columbia	tsl1	Grown at 26℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (26℃) for 7 days. To prevent possible interference of ivory leaves	NA	The newly developed true leaves of tsl1 plants turned pale green. the plastids in the tsl1 mutant plants were small and abnormally shaped, including less developed thylakoid membranes and starch granules compared to that in the WT plants.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 0 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	50.00%	Medium	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 0 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	45.00%	Medium	Similar	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 1.5 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	28.57%	Low	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 1.5 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	35.00%	Low	Similar	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 3 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	19.05%	Poor	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 3 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	21.95%	Low	Similar	32505546

Arabidopsis thaliana

NA

Chloroplast

5

CDS

G-to-A

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	WT	Grown at 22℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	88.89%	High	None	32505546
Columbia	tsl1	Grown at 22℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	NA	tsl1, grew as normally as wild-type (WT) plants	Leaf	NA	RT-PCR and sequencing	86.96%	High	Similar	32505546
Columbia	Line 3/tsl1	Grown at 22℃	For the complementation of tsl1 mutant, wild-type genomic DNA sequences of AT5G67570 including the upstream promoter sequences were amplified with PCR and inserted into pCAMBIA1300. The construct was	NA	Line 3/tsl1, grew as normally as wild-type (WT) plants	Leaf	NA	RT-PCR and sequencing	92.31%	High	Restored	32505546
Columbia	WT	Grown at 29℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (29℃) for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	81.82%	High	None	32505546
Columbia	tsl1	Grown at 29℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevatedtemperature conditions (29℃) for 7 days.	NA	The newly developed true leaves of tsl1 plants turned and uniformly white.	Leaf	NA	RT-PCR and sequencing	22.22%	Low	Decreased	32505546
Columbia	Line 3/tsl1	Grown at 29℃	For the complementation of tsl1 mutant, wild-type genomic DNA sequences of AT5G67570 including the upstream promoter sequences were amplified with PCR and inserted into pCAMBIA1300. The construct was	NA	NA	Leaf	NA	RT-PCR and sequencing	85.19%	High	Restored	32505546
Columbia	WT	Grown at 26℃	Wild-type (WT) plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (26℃) for 7 days.	Non-mutant	All the leaves of WT plants were green	Leaf	NA	RT-PCR and sequencing	100.00%	Complete	None	32505546
Columbia	tsl1	Grown at 26℃	tsl1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growth chamber under elevated temperature conditions (26℃) for 7 days. To prevent possible interference of ivory leaves	NA	The newly developed true leaves of tsl1 plants turned pale green. the plastids in the tsl1 mutant plants were small and abnormally shaped, including less developed thylakoid membranes and starch granules compared to that in the WT plants.	Leaf	NA	RT-PCR and sequencing	23.53%	Low	Decreased	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 0 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	87.80%	High	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 0 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	87.50%	High	Similar	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 1.5 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	78.95%	High	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 1.5 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	81.82%	High	Similar	32505546
Columbia	WT	Heat shock	Wild-type (WT) plants were grown at 22℃ for 8 days and then subjected to heat stress treatment(35℃) for 3 hour.	Non-mutant	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	73.53%	High	None	32505546
Columbia	tsl1	Heat shock	tsl1 mutant plants were grown at 22℃ for 8 days and then subjected to heat stress treatment (35℃) for 3 hour.	NA	All of the tested tissues appeared green before or after heat stress	Leaf	8-day-old	RT-PCR and sequencing	60.00%	High	Decreased	32505546

Last update: Jul 2021 (version 1.0)