Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Seedling | NA | PCR products were directly sequenced | 100.00% | Complete | None | 41130265 |
| Nipponbare | cr-wpr-1 | A 1-nucleotide insertion | NA | Homozygous; Knockout | A severe albino phenotype even at the seedling stage | Seedling | NA | PCR products were directly sequenced | 50.00% | Medium | Decreased | 41130265 |
| Huanghuazhan | HHZ | Control | Control | No mutant | Normal | Rachis | NA | PCR products were directly sequenced | 100.00% | Complete | Similar | 41130265 |
| Huanghuazhan | NIL-wpr | A near isogenic line | NA | No mutant | Yellow-green flag leaf sheath and white panicle rachis during heading; Transpiration rate, net photosynthesis rate, and stomatal conductance were not significantly compromised compared to that of wild-type HHZ; The yield-related agronomic traits, including plant height, number of panicles per plant, grain number per panicle, the seed-setting rate, and grain yield per plant, all showed insignificantly different results between NIL-wpr and HHZ plants | Rachis | NA | PCR products were directly sequenced | 90.14% | High | Similar | 41130265 |
| IRIS_313-11 713/IRGC 127445 | wpr | A inbred line | The wpr inbred line used in this study (IRIS_313-11 713/IRGC 127445) was selected from 3 K rice germplasms collection and collected from Nigeria | No mutant | Higher plant height and fewer tillers and was genetically classified into intermediate rice type; The flag leaf sheath (FLS) of wpr was yellow-green before heading, while panicle rachis (PR) was white in wpr during the heading stage | Rachis | NA | PCR products were directly sequenced | 82.00% | High | Decreased | 41130265 |
| Nipponbare | WT | Control | Control | No mutant | Normal | Seedling | NA | PCR products were directly sequenced | 69.00% | High | None | 41130265 |
| Nipponbare | cr-wpr-1 | A 1-nucleotide insertion | NA | Homozygous; Knockout | A severe albino phenotype even at the seedling stage | Seedling | NA | PCR products were directly sequenced | 35.00% | Low | Decreased | 41130265 |
| Huanghuazhan | HHZ | Control | Control | No mutant | Normal | Rachis | NA | PCR products were directly sequenced | 100.00% | Complete | Increased | 41130265 |
| Huanghuazhan | NIL-wpr | A near isogenic line | NA | No mutant | Yellow-green flag leaf sheath and white panicle rachis during heading; Transpiration rate, net photosynthesis rate, and stomatal conductance were not significantly compromised compared to that of wild-type HHZ; The yield-related agronomic traits, including plant height, number of panicles per plant, grain number per panicle, the seed-setting rate, and grain yield per plant, all showed insignificantly different results between NIL-wpr and HHZ plants | Rachis | NA | PCR products were directly sequenced | 87.00% | High | Increased | 41130265 |
| IRIS_313-11 713/IRGC 127445 | wpr | A inbred line | The wpr inbred line used in this study (IRIS_313-11 713/IRGC 127445) was selected from 3 K rice germplasms collection and collected from Nigeria | No mutant | Higher plant height and fewer tillers and was genetically classified into intermediate rice type; The flag leaf sheath (FLS) of wpr was yellow-green before heading, while panicle rachis (PR) was white in wpr during the heading stage | Rachis | NA | PCR products were directly sequenced | 87.00% | High | Increased | 41130265 |
| Nipponbare | WT | Control | Control | No mutant | Normal | Seedling | NA | PCR products were directly sequenced | 86.00% | High | None | 41130265 |
| Nipponbare | cr-wpr-1 | A 1-nucleotide insertion | NA | Homozygous; Knockout | A severe albino phenotype even at the seedling stage | Seedling | NA | PCR products were directly sequenced | 61.00% | High | Decreased | 41130265 |
| Huanghuazhan | HHZ | Control | Control | No mutant | Normal | Rachis | NA | PCR products were directly sequenced | 86.00% | High | Similar | 41130265 |
| Huanghuazhan | NIL-wpr | A near isogenic line | NA | No mutant | Yellow-green flag leaf sheath and white panicle rachis during heading; Transpiration rate, net photosynthesis rate, and stomatal conductance were not significantly compromised compared to that of wild-type HHZ; The yield-related agronomic traits, including plant height, number of panicles per plant, grain number per panicle, the seed-setting rate, and grain yield per plant, all showed insignificantly different results between NIL-wpr and HHZ plants | Rachis | NA | PCR products were directly sequenced | 79.00% | High | Similar | 41130265 |
| IRIS_313-11 713/IRGC 127445 | wpr | A inbred line | The wpr inbred line used in this study (IRIS_313-11 713/IRGC 127445) was selected from 3 K rice germplasms collection and collected from Nigeria | No mutant | Higher plant height and fewer tillers and was genetically classified into intermediate rice type; The flag leaf sheath (FLS) of wpr was yellow-green before heading, while panicle rachis (PR) was white in wpr during the heading stage | Rachis | NA | PCR products were directly sequenced | 81.00% | High | Similar | 41130265 |
