Accession PRJCA007961
Title Genomic DNA Contamination in RNA-seq
Relevance Medical
Data types Transcriptome or Gene expression
Raw sequence reads
Organisms Homo sapiens
Description Genomic DNA (gDNA) contamination exists in gene quantification technologies, yet the RNA-seq studies put little attention on it. gDNA contamination would lead to false results, however, the impacts of gDNA contamination for RNA-seq were still unknown. To find out the impacts of gDNA on gene expression in RNA-seq, we designed an RNA-seq experiment adding different concentrations of gDNA to extracted total RNA.
Sample scope Monoisolate
Release date 2022-08-05
Publication
PubMed ID Article title Journal name DOI Year
35922750 Genes expressed at low levels raise false discovery rates in RNA samples contaminated with genomic DNA BMC Genomics 10.1186/s12864-022-08785-1 2022
Grants
Agency program Grant ID Grant title
No funding support
Submitter Ying Yu (ying_yu@fudan.edu.cn)
Organization Fudan University
Submission date 2022-01-14

Project Data

Resource name Description
BioSample (1) -
SAMC2014904 Human lymphoblast cell