Accession |
PRJCA007961 |
Title |
Genomic DNA Contamination in RNA-seq |
Relevance |
Medical |
Data types |
Transcriptome or Gene expression
Raw sequence reads
|
Organisms |
Homo sapiens
|
Description |
Genomic DNA (gDNA) contamination exists in gene quantification technologies, yet the RNA-seq studies put little attention on it. gDNA contamination would lead to false results, however, the impacts of gDNA contamination for RNA-seq were still unknown. To find out the impacts of gDNA on gene expression in RNA-seq, we designed an RNA-seq experiment adding different concentrations of gDNA to extracted total RNA. |
Sample scope |
Monoisolate |
Release date |
2022-08-05 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
35922750
|
Genes expressed at low levels raise false discovery rates in RNA samples contaminated with genomic DNA
|
BMC Genomics
|
10.1186/s12864-022-08785-1
|
2022
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
No funding support
|
|
|
|
|
Submitter |
Ying
Yu (ying_yu@fudan.edu.cn)
|
Organization |
Fudan University |
Submission date |
2022-01-14 |