e.g., HRA000087; HRA000150
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HRA001834
Title:
Genomic DNA Contamination in RNA-seq
Release date:
2022-08-05
Description:
Genomic DNA (gDNA) contamination exists in gene quantification technologies, yet the RNA-seq studies put little attention on it. gDNA contamination would lead to false results, however, the impacts of gDNA contamination for RNA-seq were still unknown. To find out the impacts of gDNA on gene expression in RNA-seq, we designed an RNA-seq experiment adding different concentrations of gDNA to extracted total RNA.
Data Accessibility:   
Open access
BioProject:
PRJCA007961
Study type:
Cell line related study
Dataset Download
MetaData:
HRA001834.xlsx
Files:
HTTPS：https://download.cncb.ac.cn/gsa-human/HRA001834
Aspera Command Line:  Help   
FTP：ftp://download.big.ac.cn/gsa-human/HRA001834

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Individuals & samples
Individual Accession
Individual Name
Gender
Sample Accession
Sample Name
Sample Description
Individual Accession
Individual Name
Gender
Sample Accession
Sample Name
Sample Description
HRI190546GM12248maleHRS335101Human lymphoblast cellThe human lymphoblast cells were used to extract DNA and RNA
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Files
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Run accession
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Experiment
Experiment title
Platform
Sample
Run accession
Files
Experiment
Experiment title
Platform
Sample
HRR589587HRR589587_f1.fastq.gz (1.59 GB)HRX426764Polyadenylated transcripts enriched library with 0.01% gDNA adding to DNase treated total RNA replicate 1Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589587HRR589587_r2.fastq.gz (1.62 GB)HRX426764Polyadenylated transcripts enriched library with 0.01% gDNA adding to DNase treated total RNA replicate 1Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589588HRR589588_f1.fastq.gz (1.44 GB)HRX426765Polyadenylated transcripts enriched library with 0.01% gDNA adding to DNase treated total RNA replicate 2Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589588HRR589588_r2.fastq.gz (1.47 GB)HRX426765Polyadenylated transcripts enriched library with 0.01% gDNA adding to DNase treated total RNA replicate 2Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589589HRR589589_f1.fastq.gz (1.53 GB)HRX426766Polyadenylated transcripts enriched library with 0.01% gDNA adding to DNase treated total RNA replicate 3Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589589HRR589589_r2.fastq.gz (1.56 GB)HRX426766Polyadenylated transcripts enriched library with 0.01% gDNA adding to DNase treated total RNA replicate 3Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589590HRR589590_f1.fastq.gz (1.43 GB)HRX426767Polyadenylated transcripts enriched library with 0.1% gDNA adding to DNase treated total RNA replicate 1Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589590HRR589590_r2.fastq.gz (1.46 GB)HRX426767Polyadenylated transcripts enriched library with 0.1% gDNA adding to DNase treated total RNA replicate 1Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589591HRR589591_f1.fastq.gz (1.62 GB)HRX426768Polyadenylated transcripts enriched library with 0.1% gDNA adding to DNase treated total RNA replicate 2Illumina HiSeq 2000HRS335101 (SAMC2014904)
HRR589591HRR589591_r2.fastq.gz (1.66 GB)HRX426768Polyadenylated transcripts enriched library with 0.1% gDNA adding to DNase treated total RNA replicate 2Illumina HiSeq 2000HRS335101 (SAMC2014904)
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Submitter:   Yu Ying / ying_yu@fudan.edu.cn
Organization:   Fudan University
Submission date:   2022-01-14