Database Commons
Database Commons

a catalog of worldwide biological databases

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General information

Full name: Webbased Interactive Omics visualizatioN
Description: The WIlsON app is intended to interpret all types of quantitative data (e.g. multi-omics) which can be broken down to a key feature (such as genes or proteins) and assigned text columns and/or numeric values. It is designed to support common experimental designs by making use of individual data levels resulting from primary analysis.
Year founded: 2022
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Real time : Checking...
Country/Region: Germany

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Contact information

University/Institution: Goethe University
Address: Edinger Institute (Institute of Neurology), University Hospital, Goethe University, 60528 Frankfurt, Germany
Country/Region: Germany
Contact name (PI/Team): Kavi Devraj
Contact email (PI/Helpdesk):


Profiling the neurovascular unit unveils detrimental effects of osteopontin on the blood-brain barrier in acute ischemic stroke. [PMID: 35752654]
Daniel Spitzer, Sylvaine Guérit, Tim Puetz, Maryam I Khel, Moritz Armbrust, Maika Dunst, Jadranka Macas, Jenny Zinke, Gayatri Devraj, Xiaoxiong Jia, Florian Croll, Kathleen Sommer, Katharina Filipski, Thomas M Freiman, Mario Looso, Stefan Günther, Mariangela Di Tacchio, Karl-Heinz Plate, Yvonne Reiss, Stefan Liebner, Patrick N Harter, Kavi Devraj

Blood-brain barrier (BBB) dysfunction, characterized by degradation of BBB junctional proteins and increased permeability, is a crucial pathophysiological feature of acute ischemic stroke. Dysregulation of multiple neurovascular unit (NVU) cell types is involved in BBB breakdown in ischemic stroke that may be further aggravated by reperfusion therapy. Therefore, therapeutic co-targeting of dysregulated NVU cell types in acute ischemic stroke constitutes a promising strategy to preserve BBB function and improve clinical outcome. However, methods for simultaneous isolation of multiple NVU cell types from the same diseased central nervous system (CNS) tissue, crucial for the identification of therapeutic targets in dysregulated NVU cells, are lacking. Here, we present the EPAM-ia method, that facilitates simultaneous isolation and analysis of the major NVU cell types (endothelial cells, pericytes, astrocytes and microglia) for the identification of therapeutic targets in dysregulated NVU cells to improve the BBB function. Applying this method, we obtained a high yield of pure NVU cells from murine ischemic brain tissue, and generated a valuable NVU transcriptome database ( ). Dissection of the NVU transcriptome revealed Spp1, encoding for osteopontin, to be highly upregulated in all NVU cells 24 h after ischemic stroke. Upregulation of osteopontin was confirmed in stroke patients by immunostaining, which was comparable with that in mice. Therapeutic targeting by subcutaneous injection of an anti-osteopontin antibody post-ischemic stroke in mice resulted in neutralization of osteopontin expression in the NVU cell types investigated. Apart from attenuated glial activation, osteopontin neutralization was associated with BBB preservation along with decreased brain edema and reduced risk for hemorrhagic transformation, resulting in improved neurological outcome and survival. This was supported by BBB-impairing effects of osteopontin in vitro. The clinical significance of these findings is that anti-osteopontin antibody therapy might augment current approved reperfusion therapies in acute ischemic stroke by minimizing deleterious effects of ischemia-induced BBB disruption.

Acta Neuropathol. 2022:144(2) | 11 Citations (from Europe PMC, 2023-09-23)



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Created on: 2023-08-28
Curated by:
Yue Qi [2023-09-12]
Yuanyuan Cheng [2023-09-06]
Jane Young [2023-08-28]