| Accession | CRX020495 |
| Organism | Homo sapiens |
| Title | human siOCT4#1_8cell_RNA-seq |
| BioProject | PRJCA000484 |
| BioSample | SAMC017361 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| human siOCT4#1_8cell_RNA-seq |
The embryos were lysed directly and used for cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, 634888). The cDNA samples were fragmented to 200-400bp by Covaris sonicator (Covaris). Sequencing libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2018-03-09 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR024477 |
CRR024477_f1.fq.gz
CRR024477_r2.fq.gz
|
5,427.8
5,565.35
|
|
| Submitter | Lei Gao (gaol@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2017-11-12 |
|---|
