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Total RNA were isolated using TRIZOL, and the RiboErase Kit was employed to remove ribosomal RNA. Then, total RNA was divided into two groups for construction of RNase R and Atailing/RNase R libraries. RNase R treated RNA was reverse transcribed using random hexamers and SMARTer or Maxima reverse transcriptase. For each group, the cDNAs were divided into two aliquots, and one part was treated with RNase H. Finally, Agencourt AMPure XP magnetic beads were used for size selection to approximate 400bp, 600bp and 1kb, respectively. |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
SINGLE
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