| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
crosslinking cells with f digesting DNA with a suitable 4-cutter restriction enzyme Dpn II, filling ends and mark with biotin, ligating the resulting blunt-end fragments, purification and random shearing DNA into 300-500 bp fragments.After quality control test of the libraries using Qubit 2.0, an Agilent 2100 instrument (Agilent Technologies, CA, USA) and q-PCR, 150 bp PE sequencing of the Hi-C library were performed on the Illumina Novaseq 6000 platform. |
Hi-C |
GENOMIC |
PCR |
PAIRED
|
|
