Basic Information

Mus musculus
Accession: ICG00161
Latin Name: Mus musculus
Common Name: Mouse
Taxonomy ID: 10090
Description: Mus musculus is a small mammal of the order Rodentia, characteristically having a pointed snout, small rounded ears, and a long naked or almost hairless tail. It is one of the most numerous species of the genus Mus. Although a wild animal, the house mouse mainly lives in association with humans.

Gene Summary

MicroRNA

ICG00161-14
ICG00161-13

Circular RNA

ICG00161-14

mRNA

ICG00161-7
ICG00161-6
ICG00161-9
ICG00161-8
ICG00161-3
ICG00161-2
ICG00161-5
ICG00161-14
ICG00161-4
ICG00161-1
ICG00161-12
ICG00161-11
ICG00161-10

Studies (14)

ICG00161-1

Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Tbp TATA-binding Protein
Dextran Sodium Sulfate Colitis
NM_013684
F: ACCGTGAATCTTGGCTGTAAAC
R: GCAGCAAATCGCTTGGGATTA
86 60.8~61.3 SYBR
Eef2 Elongation Factor
Dextran Sodium Sulfate Colitis
NM_007907.2
F: TGTCAGTCATCGCCCATGTG
R: CATCCTTGCGAGTGTCAGTGA
123 62.1~62.2 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Jean-Eric Ghia
Email: Jean-Eric.Ghia@umanitoba.ca
Institution: Immunology, University of Manitoba, Winnipeg, MB, Canada

Reference

Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis.
Eissa N, Hussein H, Wang H, Rabbi MF, Bernstein CN, Ghia JE.
PloS one. 2016-05-31

ICG00161-10

Developmental Stage

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Ppia Peptidylprolyl Isomerase
During Testis Development
NM_008907
F: TGTGCCAGGGTGGTGACTT
R: CGTTTGTGTTTGGTCCAGCAT
144 60 SYBR
Gapdh Glyceraldehyde-3-Phosphate Dehydrogenase
During Testis Development
NM_008084
F: TGTGTCCGTCGTGGATCTGA
R: TTGCTGTTGAAGTCGCAGGAG
150 60 SYBR
Actb Actin
During Testis Development
NM_007393
F: CATCCGTAAAGACCTCTATGCCAAC
R: ATGGAGCCACCGATCCACA
171 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Wen Zhen Lin
Email: linwenzhen@msn.com
Institution: Department of Biochemistry and Molecular Biology, School of Preclinical Sciences, Guangxi Medical University, Nanning 530021, Guangxi, China

Reference

Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development.
Gong ZK, Wang SJ, Huang YQ, Zhao RQ, Zhu QF, Lin WZ.
Molecular genetics and genomics : MGG. 2014-06-22

ICG00161-11

Cell Types
Intestinal Epithelial Cells
Epithelial Cell Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Ppib Peptidylprolyl Isomerase
Proliferating Intestinal Compartments
Iec6 Cell Line
NM_011149.2
F: CACCAATGGCTCACAGTTCTT
R: ATGACATCCTTCAGTGGCTTG
156 60 SYBR
Ppia Peptidylprolyl Isomerase
Proliferating Intestinal Compartments
Iec6 Cell Line
NM_008907.1
F: CCACTGTCGCTTTTCGCCGC
R: TGCAAACAGCTCGAAGGAGACGC
109 60 SYBR
Gapdh Glyceraldehyde-3-Phosphate Dehydrogenase
Proliferating Intestinal Compartments
Iec6 Cell Line
XM_003945995.1
F: AGCAAGGACACTGAGCAAGAG
R: GCAGCGAACTTTATTGATGGT
152 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Maria Sirakov
Email: maria.sirakov@ulb.ac.be
Institution: Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies, Belgium

Reference

Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells.
Sirakov M, Borra M, Cambuli FM, Plateroti M.
Molecular biotechnology. 2013-07-01

ICG00161-12

Tissues
Genotypes

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Rpl27 Ribosomal Protein L27
R129 Strain
Skeletal Muscle
NM_011289
F: AAGCCGTCATCGTGAAGAACA
R: CTTGATCTTGGATCGCTTGGC
143 65 SYBR
Rer1 Retention In Endoplasmic Reticulum
R129 Strain
C57Bl/6J Strain
C57Bl/10 Strain
Skeletal Muscle
NM_026395
F: GCCTTGGGAATTTACCACCT
R: CTTCGAATGAAGGGACGAAA
137 62 SYBR
Rpl41 Ribosomal Protein L41
Keletal Muscle
NM_018860
F: GCCATGAGAGCGAAGTGG
R: CTCCTGCAGGCGTCGTAG
113 65 SYBR
Rpl7l1 Ribosomal Protein L7
C57Bl/6J Strain
C57Bl/10 Strain
Skeletal Muscle
NM_025433
F: ACGGTGGAGCCTTATGTGAC
R: TCCGTCAGAGGGACTGTCTT
110 65 SYBR
Aldoa Aldolase
C57Bl/10 Strain
Skeletal Muscle
NM_001177307
F: ACATTGCTGAAGCCCAACAT
R: ACAGGAAAGTGACCCCAGTG
136 62 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Kathryn N. North
Email: Kathryn.north@mcri.edu.au
Institution: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Reference

Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.
Thomas KC, Zheng XF, Garces Suarez F, Raftery JM, Quinlan KG, Yang N, North KN, Houweling PJ.
PloS one. 2014-02-11

ICG00161-13

Tissues
Hepatotoxicity Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
miR-16 MiRNA
Liver
MI0000844
F: CAGCCTAGCAGCACGTAAAT
R: GAGGTATTCGCACCAGAGGA
50 60 SYBR

Molecular Types:

MicroRNA

Evaluation Methods

Contact

Name: Luis M. Veggi
Email: veggi@ifise-conicet.gov.ar
Institution: IFISE, CONICET-UNR, Rosario, Argentina

Reference

Reference genes for real-time PCR quantification of microRNAs and messenger RNAs in rat models of hepatotoxicity.
Lardizábal MN, Nocito AL, Daniele SM, Ornella LA, Palatnik JF, Veggi LM.
PloS one. 2012-05-01

ICG00161-14

Forensic Research Models
Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
miR-122 MiRNA
Estimation Of Postmortem Interval (Pmi)
Heart
Liver
-
F: TGGAGTGTGACAATGGTGTTTG
R: Uni-miR qPCR primer
~85 60 SYBR
18S 18S Ribosomal RNA
Estimation Of Postmortem Interval (Pmi)
Heart
-
F: AGAAACGGCTACCACATCCA
R: CCCTCCAATGGATCCTCGTT
- 60 SYBR
LC-Ogdh Oxoglutarate Dehydrogenase
Estimation Of Postmortem Interval (Pmi)
Liver
-
F: GGTGGAAGCACAACCTAACG
R: TGAGATAATGTCAGCGGGCA
- 60 SYBR
circ-AFF1 CircRNA
Estimation Of Postmortem Interval (Pmi)
Liver
Skeletal Muscle
-
F: CAGCAAAGCTTTGAGAAACCAGA
R: CATGACCTAAGGGATATCTGGGC
- 60 SYBR
LC-LRP6 LDL Receptor Related Protein
Estimation Of Postmortem Interval (Pmi)
Skeletal Muscle
-
F: CGGCCCCTTTGTTGCTTTAT
R: TTCGCTGACATCACTCCAGT
- 60 SYBR
miR-133a MiRNA
Estimation Of Postmortem Interval (Pmi)
Heart
Skeletal Muscle
-
F: TTTGGTCCCCTTCAACCAGCTG
R: Uni-miR qPCR primer
~85 60 SYBR

Molecular Types:

Circular RNA @@ MicroRNA

Evaluation Methods

Contact

Name: Yiwen Shen
Email: shenyiwen@fudan.edu.cn
Institution: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, 138 Yixueyuan Road, Xuhui District, Shanghai 200032, China

Reference

Evaluating the potential of housekeeping genes, rRNAs, snRNAs, microRNAs and circRNAs as reference genes for the estimation of PMI.
Tu C, Du T, Shao C, Liu Z, Li L, Shen Y.
Forensic science, medicine, and pathology. 2018-04-24

ICG00161-2

Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
HPRT Hypoxanthine Phosphoribosyltransferase
Mammary Gland
NM_013556
F: CCTAAGATGAGCGCAAGTTGAA
R: CCACAGGACTAGAACACCTGCTAA
86 60 SYBR
RPL13A Ribosomal Protein L13
Mammary Gland
NM_009438
F: CCTAAGATGAGCGCAAGTTGAA
R: CCACAGGACTAGAACACCTGCTAA
168 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Mammary Gland
NM_008084
F: TGTGTCCGTCGTGGATCTGA
R: TTGCTGTTGAAGTCGCAGGAG
150 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Y.L. Wang
Email: ylwang2001@yahoo.cn
Institution: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China

Reference

Selection and use of reference genes in mouse mammary glands.
Han LQ, Yang GY, Zhu HS, Wang YY, Wang LF, Guo YJ, Lu WF, Li HJ, Wang YL.
Genetics and molecular research : GMR. 2010-03-16

ICG00161-3

Tissues
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
RPLP0 Ribosomal Protein LP
Uterine
Early Pregnancy
Pseudopregnancy
Delayed Implantation
Artificial Decidualization
Hormonal Treatment Model
NM_007475
F: GGACCCGAGAAGACCTCCTT
R: GCACATCACTCAGAATTTCAATGG
85 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Uterine
Early Pregnancy
Pseudopregnancy
Delayed Implantation
Artificial Decidualization
Hormonal Treatment Model
NM_008084
F: TCACTGCCACCCAGAAGA
R: GACGGACACATTGGGGGTAG
186 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: AiHua Wang
Email: pflin2001n@163.com
Institution: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China

Reference

Reference gene selection for real-time quantitative PCR analysis of the mouse uterus in the peri-implantation period.
Lin P, Lan X, Chen F, Yang Y, Jin Y, Wang A.
PloS one. 2013-04-24

ICG00161-4

Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Rpl13a Ribosomal Protein L13
Cerebral Cortex During Ageing
Cortex During Ageing
Hippocampus During Ageing
NM_009438.5
F: TACGCTGTGAAGGCATCAAC
R: GGGAGGGGTTGGTATTCATC
96 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Cerebral Cortex During Ageing
Cortex During Ageing
Cerebellum During Ageing
NM_008084.3
F: TGCGACTTCAACAGCAACTC
R: ATGTAGGCAATGAGGTCCAC
143 60 SYBR
Ppib Peptidylprolyl Isomerase
Striatum During Ageing
Cerebellum During Ageing
NM_011149.2
F: CAGCAAGTTCCATCGTGTCA
R: GATGCTCTTTCCTCCTGTGC
85 60 SYBR
Hprt Hypoxanthine Phosphoribosyltransferase
Hippocampus During Ageing
Cerebellum During Ageing
NM_013556.2
F: CTTTGCTGACCTGCTGGATT
R: TATGTCCCCCGTTGACTGAT
121 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: B.D.Roussel
Email: broussel@cyceron.fr
Institution: INSERM, INSERM U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, GIP CYCERON, University Caen Basse Normandie, boulevard Becquerel, 14074 Caen, France

Reference

Normalization of Reverse Transcription Quantitative PCR Data During Ageing in Distinct Cerebral Structures.
Bruckert G, Vivien D, Docagne F, Roussel BD.
Molecular neurobiology. 2015-02-07

ICG00161-5

Tissues
Lung Research Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Tuba1a Tubulin
Lung
Different Stages Of Lung Development
NM_011653
F: CCGCGAAGCAGCAACCAT
R: CCAGGTCTACGAACACTGCC
227 60.39~61.43 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Guillermo Barreto
Email: guillermo.barreto@mpi-bn.mpg.de
Institution: LOEWE Research Group Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research, Parkstraße 1, 61231 Bad Nauheim, Germany

Reference

Validation of Tuba1a as appropriate internal control for normalization of gene expression analysis during mouse lung development.
Mehta A, Dobersch S, Dammann RH, Bellusci S, Ilinskaya ON, Braun T, Barreto G.
International journal of molecular sciences. 2015-02-25

ICG00161-6

Cell Types
Intestine Research Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
GUSB Glyceraldehyde-3-Phosphate Dehydrogenase
Eepithelial Cells
Intestine
Iec6 Cell Line
NM_010368
F: CCGATTATCCAGAGCGAGTATG
R: CTCAGCGGTGACTGGTTCG
197 55 SYBR
TBP TATA-binding Protein
Eepithelial Cells
Intestine
Iec6 Cell Line
NM_008907
F: CAAACCCAGAATTGTTCTCCTT
R: ATGTGGTCTTCCTGAATCCCT
131 5 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Yongping Su
Email: suyp2003@yahoo.com.cn
Institution: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Shapingba District, Chongqing 400038, China

Reference

Normalizing genes for real-time polymerase chain reaction in epithelial and nonepithelial cells of mouse small intestine.
Wang F, Wang J, Liu D, Su Y.
Analytical biochemistry. 2009-12-28

ICG00161-7

Developmental Stage
Embryo Research Model
Strains

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
h2afz Histone
B6D2F-1 Strain
C57Bl/6 Strains
Blastocyst Embryonic Stage
Ksom Culture
Czb Culture
C57Bl/6 Strain
Zygote Stage Embryo
2-Cell Stage
4-Cell Stage
8-Cell Stage
M16 Culture
NM_016750
F: GTGGACTGTATCTCTGTGAA
R: GGTTGGTTGGAAGGCTAA
89 60 SYBR
sptbn Pectrin
Icr Strains
NM_175836
F: TCTAATGGTTACTTGCTTGT
R: CAATAGTTACAGTGACAGAGA
101 55 SYBR
wrnip Helicase
M16 Culture
B6D2F-1 Strain
C57Bl/6 Strain
Czb Culture
Blastocyst Stage Embryos
M16 Culture
2-Cell Stage
4-Cell Stage
8-Cell Stage
NM_030215
F: ATGAGTAGGATGCTTGTA
R: TAACCACCTCCATCTATG
130 56 SYBR
ywhaz Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta Polypeptide
Zygote
Molular Embryonic Stage
Ksom Culture
Czb Culture
NM_001253807
F: CAGTAGATGGAGAAAGATTTGC
R: GGGACAATTAGGGAAGTAAGT
92 60 SYBR
tgfb1 Transforming Growth Factor
2-Cell Stage
Ksom Culture
NM_011577
F: TATACTGAGACACCTTGG
R: GTGATAGTCCTGAATAATTTG
83 55 SYBR
18s 18S Ribosomal RNA
2-Cell Stage
4-Cell Stage
8-Cell Stage
Ksom Culture
M16 Culture
X00686
F: CGCCGCTAGAGGTGAAATTCT
R: CGAACCTCCGACTTTCGTTCT
102 60 SYBR
actb Actin
M16 Culture
NM_007393
F: ATCTTCCGCCTTAATACT
R: GCCTTCATACATCAAGTT
98 56 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Jin-Hoi Kim
Email: jhkim541@konkuk.ac.kr
Institution: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Republic of Korea

Reference

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR).
Jeong JK, Kang MH, Gurunathan S, Cho SG, Park C, Seo HG, Kim JH.
BMC research notes. 2014-09-25

ICG00161-8

Developmental Stage
Stem Cells Research Model
Cell Types

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
Pgk1 Phosphoglycerate Kinase
Es Stem Cells
Ts Stem Cells
Xen Stem Cells
In Vitro Differentiation
Embryonic Stem Cells
Trophectoderm Stem Cells
NM_008828
F: CTGACTTTGGACAAGCTGGACG
R: GCAGCCTTGATCCTTTGGTTG
110 60 SYBR
Sdha Succinate Dehydrogenase Complex
Es Stem Cells
Ts Stem Cells
Xen Stem Cells
In Vitro Differentiation
Embryonic Stem Cells
Trophectoderm Stem Cells
BC011301
F: GCTCCTGCCTCTGTGGTTGA
R: AGCAACACCGATGAGCCTG
134 60 SYBR
Tbp TATA-binding Protein
Es Stem Cells
Ts Stem Cells
Xen Stem Cells
In Vitro Differentiation
Embryonic Stem Cells
Trophectoderm Stem Cells
NM_013684
F: GAAGAACAATCCAGACTAGCAGCA
R: CCTTATAGGGAACTTCACATCACAG
127 60 SYBR
Ywhaz Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta Polypeptide
In Vitro Differentiation
Embryonic Stem Cells
Trophectoderm Stem Cells
NM_013684
F: TTGATCCCCAATGCTTCGC
R: CAGCAACCTCGGCCAAGTAA
88 60 SYBR
Hk2 Hexokinase
In Vitro Differentiation
Embryonic Stem Cells
Trophectoderm Stem Cells
NM_011740
F: CCCTGTGAAGATGTTGCCCAC
R: TGCCCATGTACTCAAGGAAGT
251 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Michael C. Golding
Email: mgolding@cvm.tamu.edu
Institution: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America

Reference

Selection of stable reference genes for quantitative rt-PCR comparisons of mouse embryonic and extra-embryonic stem cells.
Veazey KJ, Golding MC.
PloS one. 2011-11-10

ICG00161-9

Tissues
Abiotic Stresses
Obesity Research Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
B2M Microglobulin
Liver
Msg Injection
Developed Central Obesity
NM_009735
F: GGTCTTTCTGGTGCTTGTCTCA
R: GTTCGGCTTCCCATTCTCC
103 55-60 SYBR
18S 18S Ribosomal RNA
Liver
Msg Injection
Developed Central Obesity
NR_003278
F: GGCCGTTCTTAGTTGGTGGAGCG
R: CTGAACGCCACTTGTCCCTC
133 55-60 SYBR
HPRT1 Hypoxanthine Phosphoribosyltransferase
Liver
Msg Injection
Developed Central Obesity
NM_013556
F: CAGTCCCAGCGTCGTGATTA
R: GGCCTCCCATCTCCTTCATG
167 55-60 SYBR
RPlP0 Ribosomal Protein LP
Small Intestine
Msg Injection
Developed Central Obesity
NM_007475
F: GATGGGCAACTGTACCTGACTG
R: CTGGGCTCCTCTTGGAATG
136 55-60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Petra Matoušková
Email: matousp7@faf.cuni.cz
Institution: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Reference

Reference genes for real-time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity.
Matoušková P, Bártíková H, Boušová I, Hanušová V, Szotáková B, Skálová L.
PloS one. 2014-01-17