The variant sites were graded into I-III according to the number of mutations in high-quality sequences and the density distribution of mutations (population frequency of class I is greater than 0.05, which indicates it is more credible; class II variant sites fall in high-density areas; population frequency of class III is less than 0.05, indicating its low reliability). Specific definitions are detailed in the table below.
| Population Frequency |
High-density Mutated Region |
Quality Grading |
| >=0.05 |
NO |
I |
| >=0.05 |
Yes(caused by multiple samples) |
I |
| >=0.05 |
Yes(caused by single sample) |
II |
| 0.01~0.05 |
NO |
II |
| 0.01~0.05 |
Yes(caused by multiple samples) |
II |
| 0.01~0.05 |
Yes(caused by single sample) |
III |
| <0.01 |
|
III |