Genome Sequences
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The variant sites were graded into I-III according to the number of mutations in high-quality sequences and the density distribution of mutations (population frequency of class I is greater than 0.05, which indicates it is more credible; class II variant sites fall in high-density areas; population frequency of class III is less than 0.05, indicating its low reliability). Specific definitions are detailed in the table below.
| Population Frequency | High-density Mutated Region | Quality Grading |
|---|---|---|
| >=0.05 | NO | I |
| >=0.05 | Yes（caused by multiple samples） | I |
| >=0.05 | Yes（caused by single sample） | II |
| 0.01~0.05 | NO | II |
| 0.01~0.05 | Yes（caused by multiple samples） | II |
| 0.01~0.05 | Yes（caused by single sample） | III |
| <0.01 | III |