OMIX

Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers

OMIX656

1Summary
Title Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers
Description Single-stranded guanine-rich RNA sequences have a propensity to fold into compact G-quadruplexes (RG4s). The conformational transitions of these molecules provide an important way to regulate their biological functions. Here, we examined the stability and conformation of an RG4-forming sequence identified near the end of human telomerase RNA. We found that a proximal single-stranded (ss) RNA significantly impairs RG4 stability at physiological K+ concentrations, resulting in a reduced RG4 rupture force of 24.4 pN and easier accessibility of the G-rich sequence. The destabilizing effect requires a minimum of six nucleotides of ssRNA and is effective at either end of RG4. Remarkably, this RG4-forming sequence, under the influence of such a proximal ssRNA, exhibits interconversions between at least three less stable RG4 conformers that might represent potential intermediates along its folding/unfolding pathway.
Organism Homo sapiens
Data Type -
Data Accessibility Open-access
BioProject PRJCA006631
Release Date 2021-09-19
Submitter Xia Zhang (zhangxia@shanghaitech.edu.cn)
Organization ShanghaiTech University
Submission Date 2021-09-19
2Files & Download

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OMIX656-99-01 Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers 12564 Protein 3D Structure Data 113.49 GB rar 0

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