Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers
| Title | Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers |
|---|---|
| Description | Single-stranded guanine-rich RNA sequences have a propensity to fold into compact G-quadruplexes (RG4s). The conformational transitions of these molecules provide an important way to regulate their biological functions. Here, we examined the stability and conformation of an RG4-forming sequence identified near the end of human telomerase RNA. We found that a proximal single-stranded (ss) RNA significantly impairs RG4 stability at physiological K+ concentrations, resulting in a reduced RG4 rupture force of 24.4 pN and easier accessibility of the G-rich sequence. The destabilizing effect requires a minimum of six nucleotides of ssRNA and is effective at either end of RG4. Remarkably, this RG4-forming sequence, under the influence of such a proximal ssRNA, exhibits interconversions between at least three less stable RG4 conformers that might represent potential intermediates along its folding/unfolding pathway. |
| Organism | Homo sapiens |
| Data Type | - |
| Data Accessibility | Open-access |
| BioProject | PRJCA006631 |
| Release Date | 2021-09-19 |
| Submitter | Xia Zhang (zhangxia@shanghaitech.edu.cn) |
| Organization | ShanghaiTech University |
| Submission Date | 2021-09-19 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX656-99-01 | Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers | 12564 | Protein 3D Structure Data | 113.49 GB | rar | 0 |