Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua.

Trond Løvdal, Maria Befring Hovda, Benny Björkblom, Simon G Møller

Author Information

Trond Løvdal: Nofima Norconserv AS, Stavanger, Norway. trond.lovdal@nofima.no

PMID: 21324348 DOI: 10.1016/j.mimet.2011.01.027

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.

Azides

Colony Count, Microbial

Hot Temperature

Listeria

Microbial Viability

Polymerase Chain Reaction

Propidium

Staining and Labeling

Azides

propidium monoazide

Propidium

Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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