In order to study river health status from harmonic relationship between human and natural environment, a river health evaluation method was proposed from the aspects of ecosystem integrity and human service demand, and the understanding of river health connotation. The proposed method is based on the harmony theory and two types of river health assessment methods (the forecasting model and index evaluation). A new framework for assessing river water health was then formed from the perspective of harmony and dynamic evolution between human service demand and river ecosystem integrity. As a case study, the method and framework were applied to the Shaying River Basin, a tributary of the most polluted Huaihe River Basin in China. The health status of the river's ecosystem and its effect on the mainstream of Huaihe River were evaluated based on water ecological experiment. The results indicated that: (1) the water ecological environment in Shaying River was generally poor and showed a gradual changing pattern along the river. The river health levels were generally "medium" in the upstream but mostly "sub-disease" in the midstream and downstream, indicating that the water pollution in Shaying River were mainly concentrated in the midstream and downstream; (2) the water pollution of Shaying River had great influence on the ecosystem of Huaihe River, and the main influencing factors were TN, followed by TP and COD; (3) the natural attribute of river was transferring toward to the direction of socialization due to the increasing human activities. The stronger the human activity intervention is, the faster the transfer will be and the more river's attributes will match with human service demand. The proposed framework contributes to the research in water ecology and environment management, and the research results can serve as an important reference for basin management in Shaying River and Huaihe River.
