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Jul 05, 2022;11 (7):

The C-Terminal Repeat Units of SpaA Mediate Adhesion of to Host Cells and Regulate Its Virulence.

Chao Wu, Zhewen Zhang, Chao Kang, Qiang Zhang, Weifeng Zhu, Yadong Zhang, Hao Zhang, Jingfa Xiao, Meilin Jin
Author Information
  1. Chao Wu: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
  2. Zhewen Zhang: National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China.
  3. Chao Kang: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
  4. Qiang Zhang: College of Biomedicine and Health, Huazhong Agricultural University, Wuhan 430070, China. ORCID
  5. Weifeng Zhu: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
  6. Yadong Zhang: National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China.
  7. Hao Zhang: National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China. ORCID
  8. Jingfa Xiao: National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China. ORCID
  9. Meilin Jin: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. ORCID
PMID: 36101391 DOI: 10.3390/biology11071010

Abstract

is a causative agent of erysipelas in animals and erysipeloid in humans. However, current information regarding pathogenesis remains limited. Previously, we identified two strains, SE38 and G4T10, which were virulent and avirulent in pigs, respectively. Here, to further study the pathogenic mechanism of , we sequenced and assembled the genomes of strains SE38 and G4T10, and performed a comparative genomic analysis to identify differences or mutations in virulence-associated genes. Next, we comparatively analyzed 25 virulence-associated genes in SE38 and G4T10. Compared with that of SE38, the gene of the G4T10 strain lacked 120 bp, encoding repeat units at the C-terminal of SpaA. To examine whether these deletions or splits influence virulence, these 120 bp were successfully deleted from the gene in strain SE38 by homologous recombination. The mutant strain displayed attenuated virulence in mice and decreased adhesion to porcine iliac artery endothelial cells, which was also observed using the corresponding mutant protein SpaA'. Our results demonstrate that SpaA-mediated adhesion between and host cells is dependent on its C-terminal repeat units.

Keywords

C-terminal repeat Erysipelothrix rhusiopathiae SpaA comparative genomics virulence

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Grants

  1. 2020ABA016/Major special projects of technological innovation in Hubei Province
  2. 32170669/National Science Foundation of China
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