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Genomics, proteomics & bioinformatics
02, 2023;21 (1): 127-149.

The Jasmine (Jasminum sambac) Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates.

Gang Chen, Salma Mostafa, Zhaogeng Lu, Ran Du, Jiawen Cui, Yun Wang, Qinggang Liao, Jinkai Lu, Xinyu Mao, Bang Chang, Quan Gan, Li Wang, Zhichao Jia, Xiulian Yang, Yingfang Zhu, Jianbin Yan, Biao Jin
Author Information
  1. Gang Chen: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
  2. Salma Mostafa: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Department of Floriculture, Faculty of Agriculture, Alexandria University, Alexandria 21526, Egypt.
  3. Zhaogeng Lu: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
  4. Ran Du: Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
  5. Jiawen Cui: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  6. Yun Wang: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  7. Qinggang Liao: Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
  8. Jinkai Lu: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  9. Xinyu Mao: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  10. Bang Chang: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  11. Quan Gan: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  12. Li Wang: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  13. Zhichao Jia: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
  14. Xiulian Yang: College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China.
  15. Yingfang Zhu: Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, Kaifeng 475001, China.
  16. Jianbin Yan: Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China. Electronic address: jianbinlab@caas.cn.
  17. Biao Jin: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: bjin@yzu.edu.cn.
PMID: 36587654 DOI: 10.1016/j.gpb.2022.12.005

Abstract

Jasminum sambac (jasmine flower), a world-renowned plant appreciated for its exceptional flower fragrance, is of cultural and economic importance. However, the genetic basis of its fragrance is largely unknown. Here, we present the first de novogenome assembly of J. sambac with 550.12 Mb (scaffold N50 = 40.10 Mb) assembled into 13 pseudochromosomes. Terpene synthase (TPS) genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome. Gene clusters within the salicylic acid/benzoic acid/theobromine (SABATH) and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase (BAHD) superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds. Several key genes involved in jasmonate biosynthesis were duplicated, causing an increase in copy numbers. In addition, multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways. Furthermore, the roles of JsTPS3 in β-ocimene biosynthesis, as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis, were functionally validated. The genome assembled in this study for J. sambac offers a basic genetic resource for studying floral scent and jasmonate biosynthesis, and provides a foundation for functional genomic research and variety improvements in Jasminum.

Keywords

Flower fragrance Genome Jasmine flower Jasminum sambac Jasmonate

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MeSH Term

Jasminum
Odorants
Cyclopentanes
Flowers

Chemicals

jasmonic acid
Cyclopentanes
Journal Article Research Support, Non-U.S. Gov't

Links to CNCB-NGDC Resources

GWH: GWHAZHY00000000 (Jasminum sambac)
BioProject: PRJCA003967 (Jasminum sambac genome)
GSA: CRA005359 (Transcriptome sequencing of different tissues of Jasminum sambac)
GSA: CRA005361 (Transcriptome sequencing of flower buds and full-bloom flowers of Jasminum sambac)
GSA: CRA005366 (Transcriptome sequencing of jasmine leaves after wounding)
GSA: CRA008133 (Jasminum sambac Genome sequencing and assembly)
Article has an altmetric score of 3

Word Cloud

Created with Highcharts 10.0.0biosynthesissambacfragranceJasminumflowergenesgeneticJassembledclustersgenomeidentifiedcompoundsinvolvedjasmonateJasmineGenomeFlowerjasmineworld-renownedplantappreciatedexceptionalculturaleconomicimportanceHoweverbasislargelyunknownpresentfirstdenovogenomeassembly55012 MbscaffoldN50 = 4010 Mb13pseudochromosomesTerpenesynthaseTPSassociatedconsiderablyamplifiedformgenetandemduplicationsGenewithinsalicylicacid/benzoicacid/theobromineSABATHbenzylalcoholO-acetyltransferase/anthocyaninO-hydroxycinnamoyltransferases/anthranilateN-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline4-O-acetyltransferaseBAHDsuperfamiliesrelatedphenylpropanoid/benzenoidSeveralkeyduplicatedcausingincreasecopynumbersadditionmulti-omicsanalysesvariousaromaticmanypathwaysFurthermorerolesJsTPS3β-ocimenewellJsAOC1JsAOSjasmonicacidfunctionallyvalidatedstudyoffersbasicresourcestudyingfloralscentprovidesfoundationfunctionalgenomicresearchvarietyimprovementsProvidesInsightBiosynthesisFragrancesJasmonatesJasmonate

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