Standardization of Galleria mellonella as an Infection Model for Malassezia furfur and Malassezia pachydermatis.

Maritza Torres, Adriana Marcela Celis Ramírez
Author Information
  1. Maritza Torres: Grupo de Investigación Celular y Molecular de Microorganismos Patógenos (CeMoP), Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
  2. Adriana Marcela Celis Ramírez: Grupo de Investigación Celular y Molecular de Microorganismos Patógenos (CeMoP), Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia. acelis@uniandes.edu.co.

Abstract

Galleria mellonella larva has been widely exploited as an infection model for bacteria and fungi. Our laboratory uses this insect as a model for fungal infection caused by the genus Malassezia, in particular, systemic infections caused by Malassezia furfur and Malassezia pachydermatis, which are poorly understood. Here, we describe the G. mellonella larva inoculation process with M. furfur and M. pachydermatis and the posterior assessment of the establishment and dissemination of the infection in the larvae. This assessment was done through the evaluation of larval survival, melanization, fungal burden, hemocytes populations, and histological changes. This methodology allows for the identification of virulence patterns between Malassezia species and the impact of inoculum concentration and temperature.

Keywords

References

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MeSH Term

Animals
Malassezia
Moths
Mycoses
Larva
Reference Standards

Word Cloud

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