Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening | Leaf | 21 days | Direct Sequencing of PCR Products | 29.60% | Low | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening | Leaf | 21 days | Direct Sequencing of PCR Products | 39.90% | Low | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening | Cotyledon | 5 days | Direct Sequencing of PCR Products | 57.30% | Medium | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening | Leaf | 21 days | Direct Sequencing of PCR Products | 60.00% | High | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening | Cotyledon | 14 days | Direct Sequencing of PCR Products | 87.10% | High | Restored | 21294841 |
| Ler | WT | Control | Control | Control | Normal | Leaf | 21 days | Direct Sequencing of PCR Products | 95.40% | High | None | 21294841 |
| Ler | WT | Control | Control | Control | Normal | Leaf | 21 days | Direct Sequencing of PCR Products | 95.40% | High | None | 21294841 |
| Ler | WT | Control | Control | Control | Normal | Leaf | 21 days | Direct Sequencing of PCR Products | 95.40% | High | None | 21294841 |
| Ler | WT | Control | Control | Control | Normal | Cotyledon | 14 days | Direct Sequencing of PCR Products | 96.50% | High | None | 21294841 |
| Ler | WT | Control | Control | Control | Normal | Cotyledon | 5 days | Direct Sequencing of PCR Products | 98.50% | High | None | 21294841 |
| SALK_112251 | atecb2 | T-DNA Insertion | NA | Knockout | Mutant atecb2 displays albino cotyledons and is seedling lethal | Seedling | 14 days | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 19500301 |
| SALK_112251 | TC | Complementation | NA | NA | NA | Seedling | 14 days | Direct Sequencing of PCR Products | 86.20% | High | Restored | 19500301 |
| Col-0 | WT | Control | Control | Control | Normal | Seedling | 14 days | Direct Sequencing of PCR Products | 89.50% | High | None | 19500301 |
| NA | NA | RNA immunoprecipitation analysis | NA | NA | NA | NA | NA | NA | NA | NA | None | 28850756 |
| Col-0 | AtECB2△E/E+ | Complementation | The genomic sequence of AtECB2 lacking the E/E+ domain (AtECB2△E/E+) is introduced into atecb2/+ heterozygous plants | NA | These lines display seedling albino phenotypes similar to that of atecb2 | NA | NA | Direct Sequencing of PCR Products | 30.00% | Low | Decreased | 28850756 |
| Col-0 | WT | Control | Control | Control | Normal | NA | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 28850756 |
| Columbia (Col-0) | Col-0 leaf | Control | Control | No mutant | Normal | Leaf | 21-day-old | PCR and Sanger sequencing; Image J software | 99.48% | High | None | 39579257 |
| Columbia (Col-0) | ecb2-3 green patches | Mutant | A leaf virescence mutant firstly named 27–27 was screened from EMS mutated Col-0 M2 seeds library | Recessive | The chlorophyll content and photosystem II maximum photochemical efficiency of ecb2-3 are obviously lower than that of Col-0 and its complementation lines, respectively; The chloroplast development of ecb2-3 is also inferior to that of Col-0 and its complementation line at the observed time points using the transmission electron microscope | Patch | 21-day-old | PCR and Sanger sequencing; Image J software | 45.39% | Medium | Decreased | 39579257 |
| Columbia (Col-0) | ecb2-3 yellow patches | Mutant | A leaf virescence mutant firstly named 27–27 was screened from EMS mutated Col-0 M2 seeds library | Recessive | The chlorophyll content and photosystem II maximum photochemical efficiency of ecb2-3 are obviously lower than that of Col-0 and its complementation lines, respectively; The chloroplast development of ecb2-3 is also inferior to that of Col-0 and its complementation line at the observed time points using the transmission electron microscope | Patch | 21-day-old | PCR and Sanger sequencing; Image J software | 2.45% | Poor | Decreased | 39579257 |
| Columbia (Col-0) | Comp leaf | Complementation | For complementation, the 1936 bp promotor and 2601 bp AtECB2 gene were amplified using AtECB2-P-1936-KpnI-F/AtECB2-DNA-403-MluI-R, AtECB2-DNA-398-MluI-F/AtECB2-DNA-2601-PstI-R, and constructed into pP | Recessive | Normal | Leaf | 21-day-old | PCR and Sanger sequencing; Image J software | 99.48% | High | Restored | 39579257 |
| Columbia (Col-0) | Col-0 cotyledon | Control | Control | No mutant | Normal | Cotyledon | 7-day-old | PCR and Sanger sequencing; Image J software | 95.63% | High | None | 39579257 |
| Columbia (Col-0) | ecb2-3 cotyledon | Mutant | A leaf virescence mutant firstly named 27–27 was screened from EMS mutated Col-0 M2 seeds library | Recessive | The chlorophyll content and photosystem II maximum photochemical efficiency of ecb2-3 are obviously lower than that of Col-0 and its complementation lines, respectively; The chloroplast development of ecb2-3 is also inferior to that of Col-0 and its complementation line at the observed time points using the transmission electron microscope | Cotyledon | 7-day-old | PCR and Sanger sequencing; Image J software | 4.42% | Poor | Decreased | 39579257 |