Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening phenotype | Leaf | 21 days | Direct Sequencing of PCR Products | 29.60% | Low | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening phenotype | Leaf | 21 days | Direct Sequencing of PCR Products | 39.90% | Low | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening phenotype | Cotyledon | 5 days | Direct Sequencing of PCR Products | 57.30% | Medium | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening phenotype | Leaf | 21 days | Direct Sequencing of PCR Products | 60.00% | High | Decreased | 21294841 |
| Ler | ecb2-2 | Point mutation | The Thr500 is converted to Ile500 in the 13th PPR motif of the atECB2 protein | A weak allelic mutant | Delayed greening phenotype | Cotyledon | 14 days | Direct Sequencing of PCR Products | 87.10% | High | Restored | 21294841 |
| Ler | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 21 days | Direct Sequencing of PCR Products | 95.40% | High | None | 21294841 |
| Ler | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 21 days | Direct Sequencing of PCR Products | 95.40% | High | None | 21294841 |
| Ler | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 21 days | Direct Sequencing of PCR Products | 95.40% | High | None | 21294841 |
| Ler | WT | No treatment | No treatment | No treatment | No treatment | Cotyledon | 14 days | Direct Sequencing of PCR Products | 96.50% | High | None | 21294841 |
| Ler | WT | No treatment | No treatment | No treatment | No treatment | Cotyledon | 5 days | Direct Sequencing of PCR Products | 98.50% | High | None | 21294841 |
| ACR3p-GUS 73 | heterozygous vac1 | T-DNA Insertion | NA | Heterozygous; Null allele | Albino to pale yellow phenotype | Seedling | 2 weeks | Direct Sequencing of PCR Products | 62.00% | High | Decreased | 20143129 |
| Col-0 | WT | No treatment | No treatment | No treatment | No treatment | Seedling | 2 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 20143129 |
| SALK_112251 | atecb2 | T-DNA Insertion | NA | Knockout | Mutant atecb2 displays albino cotyledons and is seedling lethal | Seedling | 14 days | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 19500301 |
| SALK_112251 | TC | Complementation | NA | NA | NA | Seedling | 14 days | Direct Sequencing of PCR Products | 86.20% | High | Restored | 19500301 |
| Col-0 | WT | No treatment | No treatment | No treatment | No treatment | Seedling | 14 days | Direct Sequencing of PCR Products | 89.50% | High | None | 19500301 |
| NA | NA | RNA immunoprecipitation analysis | NA | NA | NA | NA | NA | NA | NA | NA | None | 28850756 |
| Col-0 | AtECB2ΔE/E+ | Complementation | The genomic sequence of AtECB2 lacking the E/E+ domain (AtECB2ΔE/E+) is introduced into atecb2/+ heterozygous plants | NA | These lines display seedling albino phenotypes similar to that of atecb2 | NA | NA | Direct Sequencing of PCR Products | 30.00% | Low | Decreased | 28850756 |
| Col-0 | WT | No treatment | No treatment | No treatment | No treatment | NA | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 28850756 |