EMB1417 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G21190

Chloroplast

accD

1568

CDS

TAIR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Leaf	NA	The RT-PCR products were sequenced directly	70.00%	High	None	42480504
NA	EMB1417-RNAi-1	Generation of inducible silencing lines	To obtain the Arabidopsis EMB1417 silencing constructs, two 300-bp regions from the coding sequence of EMB1417 gene were amplified by PCR; PCR products were cloned into the pBWA(V)HVE (Kanamycin, Kan)	Homozygous	A yellow-leaf phenotype with markedly reduced chlorophyll content	Leaf	NA	The RT-PCR products were sequenced directly	22.00%	Low	Decreased	42480504

Arabidopsis thaliana

AT4G21190

Chloroplast

clpP

559

CDS

TAIR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Leaf	NA	The RT-PCR products were sequenced directly	100.00%	Complete	None	42480504
NA	EMB1417-RNAi-1	Generation of inducible silencing lines	To obtain the Arabidopsis EMB1417 silencing constructs, two 300-bp regions from the coding sequence of EMB1417 gene were amplified by PCR; PCR products were cloned into the pBWA(V)HVE (Kanamycin, Kan)	Homozygous	A yellow-leaf phenotype with markedly reduced chlorophyll content	Leaf	NA	The RT-PCR products were sequenced directly	55.00%	Medium	Decreased	42480504

Arabidopsis thaliana

AT4G21190

Chloroplast

ndhD

CDS

TAIR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Leaf	NA	The RT-PCR products were sequenced directly	79.00%	High	None	42480504
NA	EMB1417-RNAi-1	Generation of inducible silencing lines	To obtain the Arabidopsis EMB1417 silencing constructs, two 300-bp regions from the coding sequence of EMB1417 gene were amplified by PCR; PCR products were cloned into the pBWA(V)HVE (Kanamycin, Kan)	Homozygous	A yellow-leaf phenotype with markedly reduced chlorophyll content	Leaf	NA	The RT-PCR products were sequenced directly	6.00%	Poor	Decreased	42480504

Arabidopsis thaliana

AT4G21190

Chloroplast

petL

CDS

TAIR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Leaf	NA	The RT-PCR products were sequenced directly	59.00%	Medium	None	42480504
NA	EMB1417-RNAi-1	Generation of inducible silencing lines	To obtain the Arabidopsis EMB1417 silencing constructs, two 300-bp regions from the coding sequence of EMB1417 gene were amplified by PCR; PCR products were cloned into the pBWA(V)HVE (Kanamycin, Kan)	Homozygous	A yellow-leaf phenotype with markedly reduced chlorophyll content	Leaf	NA	The RT-PCR products were sequenced directly	4.00%	Poor	Decreased	42480504

Arabidopsis thaliana

AT4G21190

Chloroplast

psbZ

CDS

TAIR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Leaf	NA	The RT-PCR products were sequenced directly	100.00%	Complete	None	42480504
NA	EMB1417-RNAi-1	Generation of inducible silencing lines	To obtain the Arabidopsis EMB1417 silencing constructs, two 300-bp regions from the coding sequence of EMB1417 gene were amplified by PCR; PCR products were cloned into the pBWA(V)HVE (Kanamycin, Kan)	Homozygous	A yellow-leaf phenotype with markedly reduced chlorophyll content	Leaf	NA	The RT-PCR products were sequenced directly	22.00%	Low	Decreased	42480504

Arabidopsis thaliana

AT4G21190

Chloroplast

rpoA

200

CDS

TAIR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Leaf	NA	The RT-PCR products were sequenced directly	100.00%	Complete	None	42480504
NA	EMB1417-RNAi-1	Generation of inducible silencing lines	To obtain the Arabidopsis EMB1417 silencing constructs, two 300-bp regions from the coding sequence of EMB1417 gene were amplified by PCR; PCR products were cloned into the pBWA(V)HVE (Kanamycin, Kan)	Homozygous	A yellow-leaf phenotype with markedly reduced chlorophyll content	Leaf	NA	The RT-PCR products were sequenced directly	27.00%	Low	Decreased	42480504

Editing Factor:	EMB1417
Synonym:	Embryo Defective 1417
Description:	Required for splicing of rpoC1 and ycf3.2 transcripts in chloroplasts; EMB1417 is indispensable for efficient C-to-U editing at multiple sites, including rpoA-200, clpP-559, petL-5, psbZ-50, accD-1568 and ndhD-2; Disruption of EMB1417 significantly also severely impairs the splicing of group II introns in ndhB, petB, and petD transcripts
Protein Family:	Pentatricopeptide repeat (PPR)
Subclass:	P
Physical Interaction:	MORF2; MORF8; MORF9; DG409; CFM3A
Construct Structure:	P
Gene ID & Species:	AT4G21190 (Arabidopsis thaliana)
Edited Gene(s):	accD clpP ndhD psbZ petL rpoA
Editing Type(s):	C-to-U (12)
Publication(s):	[1] The pentatricopeptide repeat protein EMB1417 is required for chloroplast RNA editing and intron splicing in Arabidopsis, Journal of Experimental Botany, 2026. [PMID=42480504]

Summary

Editing Details