HDS - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G60600

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	95.00%	High	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	61.00%	High	Decreased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	56.00%	Medium	Decreased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	98.00%	High	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

ndhD

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	45.00%	Medium	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	0.00%	Unedited	Absent	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	0.00%	Unedited	Absent	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	41.00%	Medium	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	93.00%	High	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	58.00%	Medium	Decreased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	44.00%	Medium	Decreased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	96.00%	High	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

petL

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	93.00%	High	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	69.00%	High	Decreased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	73.00%	High	Decreased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	97.00%	High	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

rpl23

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	82.00%	High	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	60.00%	High	Decreased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	38.00%	Low	Decreased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	92.00%	High	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	92.00%	High	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	70.00%	High	Decreased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	47.00%	Medium	Decreased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	100.00%	Complete	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

rpoC1

488

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	21.00%	Low	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	50.00%	Medium	Increased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	60.00%	High	Increased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	22.00%	Low	Restored	41025320

Arabidopsis thaliana

AT5G60600

Chloroplast

rps14

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Wild type	No treatment	No mutant	Normal	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	73.00%	High	None	41025320
SALK_046132	hds-1	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	98.00%	High	Increased	41025320
SALK_059118	hds-2	A T-DNA insertion	A T-DNA insertion in the second exon of the HDS gene	Homozygous	The homozygous hds-1 and hds-2 mutant plants both displayed a completely pale cotyledon, severely stunted development, and an early seedling-lethal phenotype under normal growth conditions	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	94.00%	High	Increased	41025320
NA	hds-1/Com1	Complementation	To generate complementary plants, the 2 kb native promoter and genomic sequence of HDS without the stop codon were amplified from Col-0 DNA using Phanta Max Master Mix (P510, Vazyme); The PCR fragment	Heterozygous	The complementary hds-1/Com plants did not show obvious growth differences compared with the WT	Seedling	10-day-old	Bulk Sanger sequencing and ribosomal RNA-depleted RNA-sequen	78.00%	High	Restored	41025320

Editing Factor:	HDS
Synonym:	4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE SYNTHASE; CSB3; CONSTITUTIVE SUBTILISIN 3; CLB4; CHLOROPLAST BIOGENESIS 4; ISPG; GCPE
Description:	A regulator of retrograde signaling, is also involved in chloroplast RNA editing
Protein Family:	Isoprenoid biosynthesis protei
Subclass:	NA
Physical Interaction:	MORF2; MORF9
Construct Structure:	NA
Gene ID & Species:	AT5G60600 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD ndhG petL rpl23 rpoA rpoC1 rps14
Editing Type(s):	C-to-U (32)
Publication(s):	[1] The isoprenoid biosynthesis enzyme HDS participates in chloroplast RNA editing, Journal of Experimental Botany, 2026. [PMID=41025320]

Summary

Editing Details