PPO1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

149

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	70.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	95.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

467

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	45.00%	Medium	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	45.00%	Medium	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	90.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

586

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	70.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	85.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

830

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	70.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

836

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	95.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

872

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	85.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	75.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	90.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

1255

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhB

1481

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	75.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	70.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhD

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	50.00%	Medium	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhD

383

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	65.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhD

674

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	65.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	90.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhD

878

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	45.00%	Medium	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	40.00%	Medium	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	95.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhD

887

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	65.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	75.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	90.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhF

290

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

ndhG

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	85.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

petL

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	65.00%	High	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	65.00%	High	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	80.00%	High	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

rpl23

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	10.00%	Poor	Decreased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	15.00%	Poor	Decreased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	55.00%	Medium	None	24497494

Arabidopsis thaliana

AT4G01690

Chloroplast

rpoC1

488

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Salk_143057	ppo1-1	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	75.00%	High	Increased	24497494
Salk_017634	ppo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous; Null mutation	Etiolated cotyledons and eventually, seedling lethality	NA	NA	Direct Sequencing of PCR Products	75.00%	High	Increased	24497494
NA	WT	No treatment	No treatment	No treatment	No treatment	NA	NA	Direct Sequencing of PCR Products	30.00%	Low	None	24497494

Editing Factor:	PPO1
Synonym:	HEMG1, PPOX
Description:	Encodes protoporphyrinogen IX Oxidase 1 (PPO1), an enzyme that catalyzes protoporphyrinogen IX into protoporphyrin IX in the tetrapyrrole biosynthetic pathway, plays an important role in editing multiple sites of plastid RNA transcripts, most of which encode subunits of the NADH dehydrogenase-like complex (NDH), in Arabidopsis thaliana.
Protein Family:	Protoporphyrinogen oxidase
Subclass:	NA
Construct Structure:	NA
Gene ID & Species:	AT4G01690 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD rpl23 rpoC1 ndhF ndhG petL
Editing Type(s):	C-to-U (54)
Publication(s):	[1] Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing, PNAS, 2014. [PMID=24497494]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details