OpenLB
Open Library of Bioscience
Advanced Search
MicrobiologyOpen
Oct, 2014;3 (5): 668-87.

Histidine kinases mediate differentiation, stress response, and pathogenicity in Magnaporthe oryzae.

Stefan Jacob, Andrew J Foster, Alexander Yemelin, Eckhard Thines
Author Information
  1. Stefan Jacob: Institute of Biotechnology and Drug Research (IBWF), Erwin-Schr��dinger-Str. 56, D-67663, Kaiserslautern, Germany.
PMID: 25103193 DOI: 10.1002/mbo3.197

Abstract

The aim of this study is a functional characterization of 10 putative histidine kinases (HIKs)-encoding genes in the phytopathogenic fungus Magnaporthe oryzae. Two HIKs were found to be required for pathogenicity in the fungus. It was found that the mutant strains ��Mohik5 and ��Mohik8 show abnormal conidial morphology and furthermore ��Mohik5 is unable to form appressoria. Both HIKs MoHik5p and MoHik8p appear to be essential for pathogenicity since the mutants fail to infect rice plants. MoSln1p and MoHik1p were previously reported to be components of the HOG pathway in M. oryzae. The ��Mosln1 mutant is more susceptible to salt stress compared to ��Mohik1, whereas ��Mohik1 appears to be stronger affected by osmotic or sugar stress. In contrast to yeast, the HOG signaling cascade in phytopathogenic fungi apparently comprises more elements. Furthermore, vegetative growth of the mutants ��Mohik5 and ��Mohik9 was found to be sensitive to hypoxia-inducing NaNO2 -treatment. Additionally, it was monitored that NaNO2 -treatment resulted in MoHog1p phosphorylation. As a consequence we assume a first simplified model for hypoxia signaling in M. oryzae including the HOG pathway and the HIKs MoHik5p and MoHik9p.

Keywords

Appressoria HOG pathway Magnaporthe oryzae conidia differentiation histidine kinase hypoxia signaling pathogenicity

References

  1. J Cell Sci. 1997 May;110 ( Pt 10):1141-5 [PMID: 9191038]
  2. Microbiol Mol Biol Rev. 1999 Jun;63(2):479-506 [PMID: 10357859]
  3. Microbiol Mol Biol Rev. 2002 Jun;66(2):300-72 [PMID: 12040128]
  4. Genes Dev. 1996 Nov 1;10(21):2696-706 [PMID: 8946911]
  5. EMBO J. 1998 Dec 1;17(23):6952-62 [PMID: 9843501]
  6. Science. 2006 Apr 28;312(5773):583-8 [PMID: 16645097]
  7. Mol Cells. 2001 Dec 31;12(3):407-11 [PMID: 11804343]
  8. Plant Cell. 1993 Nov;5(11):1575-90 [PMID: 8312740]
  9. Annu Rev Microbiol. 2003;57:177-202 [PMID: 14527276]
  10. Mol Biosyst. 2010 Apr;6(4):721-8 [PMID: 20237650]
  11. PLoS Comput Biol. 2006 Nov 3;2(11):e143 [PMID: 17083272]
  12. Cell. 1996 Sep 20;86(6):865-75 [PMID: 8808622]
  13. Bioinformatics. 2007 Nov 1;23(21):2947-8 [PMID: 17846036]
  14. Microbiology (Reading). 2006 Mar;152(Pt 3):591-604 [PMID: 16514140]
  15. J Bacteriol. 1999 Apr;181(8):2527-34 [PMID: 10198019]
  16. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12713-8 [PMID: 9770551]
  17. Appl Environ Microbiol. 2009 Jan;75(1):127-34 [PMID: 19011080]
  18. Res Microbiol. 1994 Jun-Aug;145(5-6):481-6 [PMID: 7855435]
  19. Biochem Soc Trans. 2005 Apr;33(Pt 2):384-8 [PMID: 15787612]
  20. Genome Biol. 2002 Sep 25;3(10):REVIEWS3013 [PMID: 12372152]
  21. Mol Plant Microbe Interact. 2006 Sep;19(9):1042-50 [PMID: 16941908]
  22. Chem Rev. 2001 Aug;101(8):2353-64 [PMID: 11749377]
  23. Cell. 1996 Sep 20;86(6):845-8 [PMID: 8808618]
  24. Microbiol Mol Biol Rev. 1997 Mar;61(1):17-32 [PMID: 9106362]
  25. Plant Cell. 1999 Oct;11(10):2045-58 [PMID: 10521531]
  26. Mol Biol Evol. 1987 Jul;4(4):406-25 [PMID: 3447015]
  27. Biochem J. 1997 Mar 15;322 ( Pt 3):681-92 [PMID: 9148737]
  28. Curr Genet. 2005 May;47(5):298-306 [PMID: 15776234]
  29. Nucleic Acids Res. 2010 Jan;38(Database issue):D401-7 [PMID: 19900966]
  30. Mol Biol Evol. 2000 Dec;17(12):1956-70 [PMID: 11110912]
  31. Eukaryot Cell. 2003 Dec;2(6):1151-61 [PMID: 14665450]
  32. Mol Biol Evol. 2011 Oct;28(10):2731-9 [PMID: 21546353]
  33. Sci STKE. 2001 Nov 20;2001(109):re18 [PMID: 11717470]
  34. Eukaryot Cell. 2008 Dec;7(12):2017-36 [PMID: 18952900]
  35. Adv Microb Physiol. 1999;41:139-227 [PMID: 10500846]
  36. Mol Microbiol. 1997 Jun;24(5):1039-48 [PMID: 9220010]
  37. BMC Microbiol. 2005 Jun 14;5:35 [PMID: 15955239]
  38. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5088-92 [PMID: 1350679]
  39. Mol Microbiol. 2007 Apr;64(2):293-307 [PMID: 17378924]
  40. Biochemistry. 1994 Feb 1;33(4):902-6 [PMID: 8305438]
  41. Nature. 2005 Apr 21;434(7036):980-6 [PMID: 15846337]
  42. Fungal Genet Biol. 2005 Mar;42(3):200-12 [PMID: 15707841]
  43. Curr Genet. 2008 Oct;54(4):185-95 [PMID: 18726099]
  44. Cell. 1993 Jun 4;73(5):857-71 [PMID: 8098993]
  45. Annu Rev Phytopathol. 1997;35:211-34 [PMID: 15012522]
  46. Mol Plant Microbe Interact. 2008 May;21(5):525-34 [PMID: 18393612]
  47. Mol Microbiol. 1996 Nov;22(3):405-13 [PMID: 8939425]
  48. Science. 1993 Oct 22;262(5133):539-44 [PMID: 8211181]
  49. J Biochem. 1989 Jul;106(1):5-7 [PMID: 2674113]
  50. Antimicrob Agents Chemother. 2007 Oct;51(10):3734-6 [PMID: 17698623]
  51. Infect Immun. 2001 Aug;69(8):5207-11 [PMID: 11447209]
  52. Nature. 1994 May 19;369(6477):242-5 [PMID: 8183345]
  53. J Biol Chem. 1995 Nov 10;270(45):26849-56 [PMID: 7592927]
  54. Nature. 1990 Mar 29;344(6265):395-400 [PMID: 2157156]
  55. Curr Genet. 2010 Dec;56(6):517-28 [PMID: 20848286]
  56. Mol Plant Pathol. 2012 May;13(4):414-30 [PMID: 22471698]
  57. Mol Plant Microbe Interact. 1998 Mar;11(3):199-207 [PMID: 9487695]
  58. Biochim Biophys Acta. 2001 Apr 2;1504(2-3):179-95 [PMID: 11245784]
  59. Fungal Genet Biol. 2009 Sep;46(9):667-81 [PMID: 19520179]
  60. Biochemistry. 1998 Oct 6;37(40):14038-47 [PMID: 9760239]
  61. Nature. 2001 Feb 15;409(6822):860-921 [PMID: 11237011]
  62. Eukaryot Cell. 2005 Nov;4(11):1820-8 [PMID: 16278449]
  63. Curr Opin Microbiol. 2000 Apr;3(2):165-70 [PMID: 10745001]
  64. Mol Genet Genomics. 2004 Mar;271(2):228-36 [PMID: 14752661]
  65. Clin Infect Dis. 1995 Oct;21 Suppl 2:S162-5 [PMID: 8845445]
  66. Curr Biol. 1999 Jun 3;9(11):R416-8 [PMID: 10359687]
  67. Nat Biotechnol. 1998 Sep;16(9):839-42 [PMID: 9743116]
  68. Annu Rev Biochem. 2000;69:183-215 [PMID: 10966457]
  69. Trends Biochem Sci. 2000 Jan;25(1):7-9 [PMID: 10637602]
  70. Comput Appl Biosci. 1992 Jun;8(3):275-82 [PMID: 1633570]
  71. Microbiology (Reading). 1998 Feb;144 ( Pt 2):425-432 [PMID: 9493379]
  72. PLoS One. 2012;7(8):e42520 [PMID: 22912706]

MeSH Term

Fungal Proteins
Gene Expression Regulation, Fungal
Histidine Kinase
Magnaporthe
Multigene Family
Oryza
Plant Diseases
Protein Kinases
Spores, Fungal
Virulence

Chemicals

Fungal Proteins
Protein Kinases
Histidine Kinase
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0oryzaeHIKspathogenicityHOGMagnaporthefound��Mohik5pathwaystresssignalinghistidinekinasesphytopathogenicfungusmutantMoHik5pmutantsM��Mohik1NaNO2-treatmenthypoxiadifferentiationaimstudyfunctionalcharacterization10putative-encodinggenesTworequiredstrains��Mohik8showabnormalconidialmorphologyfurthermoreunableformappressoriaMoHik8pappearessentialsincefailinfectriceplantsMoSln1pMoHik1ppreviouslyreportedcomponents��Mosln1susceptiblesaltcomparedwhereasappearsstrongeraffectedosmoticsugarcontrastyeastcascadefungiapparentlycompriseselementsFurthermorevegetativegrowth��Mohik9sensitivehypoxia-inducingAdditionallymonitoredresultedMoHog1pphosphorylationconsequenceassumefirstsimplifiedmodelincludingMoHik9pHistidinemediateresponseAppressoriaconidiakinase

Similar Articles

Cited By (31)