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Journal of nanoscience and nanotechnology
11 01, 2019;19 (11): 6907-6923.

Differential Effects of Nano TiO��� and CeO��� on Normal Human Lung Epithelial Cells .

Sheau-Fung Thai, Carlton P Jones, Garret B Nelson, Beena Vallanat, Micaela Killius, James L Crooks, William O Ward, Carl F Blackman, Jeffrey A Ross
Author Information
  1. Sheau-Fung Thai: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  2. Carlton P Jones: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  3. Garret B Nelson: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  4. Beena Vallanat: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  5. Micaela Killius: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  6. James L Crooks: Division of Biostatistics and Bioinformatics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
  7. William O Ward: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  8. Carl F Blackman: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
  9. Jeffrey A Ross: National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
PMID: 31039842 DOI: 10.1166/jnn.2019.16737

Abstract

Nano-TiO��� and nano-CeO��� are among the most widely used engineered nanoparticles (NPs). We investigated a variety of endpoints to assess the toxicity of eight of these NPs to induce potentially adverse health effects in an Human respiratory epithelial cell model. These endpoints include cytotoxicity, reactive oxygen species (ROS)/reactive nitrogen species (RNS) production, 8-hydroxy-2_-deoxyguanosine (8-oxo-dG), endogenous DNA adducts, Apurinic/apyrimidinic (AP) sites, 4-Hrdoxynonenal (4-HNE) protein adducts, Malondialdehyde (MDA) protein adducts, and genomics analysis on altered signaling pathways. Our results indicated that cytotoxicity assays are relatively insensitive, and we detected changes in other endpoints at concentrations much lower than those inducing cytotoxicity. Among the ROS-related endpoints, 8-oxo-dG is relatively more sensitive than other assays, and nano-TiO��� induced more 8-oxo-dG formation than nano-CeO���. Finally, there are many signaling pathways changes at concentrations at which no cytotoxicity was observed. These alterations in signaling pathways correlated well with toxicity that was observed at higher concentrations, and with in vivo adverse outcome pathways caused by nano-TiO��� and nano-CeO��� in experimental animals.

References

  1. Int J Mol Sci. 2010 Jun 07;11(6):2383-92 [PMID: 20640159]
  2. Int J Oncol. 2006 Dec;29(6):1437-43 [PMID: 17088982]
  3. Eur Rev Med Pharmacol Sci. 2011 May;15(5):481-508 [PMID: 21744743]
  4. J Nanosci Nanotechnol. 2004 May;4(5):521-31 [PMID: 15503438]
  5. Chem Rev. 2013 May 8;113(5):3391-406 [PMID: 23428231]
  6. Nano Lett. 2006 Aug;6(8):1794-807 [PMID: 16895376]
  7. Arch Toxicol. 2011 Jul;85(7):733-41 [PMID: 20502881]
  8. Cardiovasc Res. 2004 Aug 15;63(3):381-90 [PMID: 15276463]
  9. Exp Lung Res. 2002 Jul-Aug;28(5):361-72 [PMID: 12097230]
  10. Toxicology. 2009 Jun 16;260(1-3):37-46 [PMID: 19464567]
  11. Int J Mol Sci. 2014 Dec 03;15(12):22258-78 [PMID: 25479073]
  12. Am J Physiol Gastrointest Liver Physiol. 2009 Jun;296(6):G1324-31 [PMID: 19342509]
  13. Antioxid Redox Signal. 2007 Apr;9(4):469-81 [PMID: 17280488]
  14. J Cell Mol Med. 2009 Aug;13(8B):1866-76 [PMID: 20141610]
  15. Toxicol Sci. 2006 May;91(1):227-36 [PMID: 16495353]
  16. Cancer Res. 2009 Nov 15;69(22):8784-9 [PMID: 19887611]
  17. Nanomedicine. 2011 Apr;7(2):123-30 [PMID: 20887814]
  18. Cell Adh Migr. 2007 Jan-Mar;1(1):33-42 [PMID: 19262085]
  19. Dose Response. 2012;10(3):344-54 [PMID: 22942868]
  20. Biol Trace Elem Res. 2011 May;140(2):186-97 [PMID: 20422311]
  21. Free Radic Biol Med. 1990;9(4):315-25 [PMID: 2283087]
  22. J Food Drug Anal. 2014 Mar;22(1):64-75 [PMID: 24673904]
  23. J Nanosci Nanotechnol. 2012 Mar;12(3):1812-20 [PMID: 22754985]
  24. Science. 2006 Feb 3;311(5761):622-7 [PMID: 16456071]
  25. IARC Monogr Eval Carcinog Risks Hum. 2010;93:1-413 [PMID: 21449489]
  26. J Signal Transduct. 2012;2012:807682 [PMID: 22203898]
  27. Ital J Biochem. 1981 Sep-Oct;30(5):375-87 [PMID: 7327909]
  28. J Biochem Mol Toxicol. 2016 Jul;30(7):331-41 [PMID: 26918567]
  29. Cold Spring Harb Perspect Med. 2015 Apr 01;5(4): [PMID: 25833940]
  30. J Cell Biol. 2002 Jul 22;158(2):357-68 [PMID: 12119354]
  31. J Nanosci Nanotechnol. 2015 Jan;15(1):492-503 [PMID: 26328389]
  32. Free Radic Biol Med. 2003 Jun 15;34(12):1507-16 [PMID: 12788471]
  33. Chem Res Toxicol. 2012 Jul 16;25(7):1402-11 [PMID: 22559321]
  34. Immunol Lett. 2008 Mar 15;116(2):104-10 [PMID: 18243340]
  35. Dose Response. 2010 Aug 12;8(4):501-17 [PMID: 21191487]
  36. J Nanosci Nanotechnol. 2015 Dec;15(12):9925-37 [PMID: 26682436]
  37. Cell Signal. 2012 Dec;24(12):2360-8 [PMID: 22917534]
  38. Biochim Biophys Acta. 2012 Jul;1820(7):1102-10 [PMID: 22561002]
  39. BMB Rep. 2010 May;43(5):311-8 [PMID: 20510013]
  40. Carcinogenesis. 1991 Oct;12(10):1953-5 [PMID: 1934277]
  41. PLoS One. 2014 Jul 07;9(7):e95662 [PMID: 24999733]
  42. J Natl Cancer Inst. 1999 Nov 3;91(21):1820-8 [PMID: 10547389]
  43. Semin Immunopathol. 2016 Jul;38(4):517-34 [PMID: 27001429]
  44. J Proteomics. 2014 Aug 28;108:469-83 [PMID: 24972317]
  45. Mutagenesis. 2017 Jan;32(1):117-126 [PMID: 27838631]
  46. Biomaterials. 2010 Feb;31(5):894-9 [PMID: 19857890]
  47. Nanotoxicology. 2011 Dec;5(4):546-56 [PMID: 21142840]
  48. Circ Res. 2001 Dec 7;89(12):1104-10 [PMID: 11739274]
  49. Toxicology. 2009 Oct 1;264(1-2):110-8 [PMID: 19666077]
  50. Carcinogenesis. 1997 Oct;18(10):1955-63 [PMID: 9364006]
  51. Eur Respir J. 2009 Sep;34(3):559-67 [PMID: 19696157]
  52. Nat Med. 2012 Jul 06;18(7):1028-40 [PMID: 22772564]
  53. Mutagenesis. 2012 Sep;27(5):541-9 [PMID: 22492202]

Grants

  1. EPA999999/Intramural EPA

MeSH Term

Animals
Epithelial Cells
Humans
Lung
Reactive Oxygen Species
Titanium

Chemicals

Reactive Oxygen Species
titanium dioxide
Titanium
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Word Cloud

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