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The New phytologist
Sep, 2024;243 (5): 1742-1757.

N-terminal acetylation orchestrates glycolate-mediated ROS homeostasis to promote rice thermoresponsive growth.

Xueting Li, Huashan Tang, Ting Xu, Pengfei Wang, Fangfang Ma, Haifang Wei, Zi Fang, Xiaoyan Wu, Yanan Wang, Yongbiao Xue, Biyao Zhang
Author Information
  1. Xueting Li: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. ORCID
  2. Huashan Tang: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  3. Ting Xu: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. ORCID
  4. Pengfei Wang: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  5. Fangfang Ma: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  6. Haifang Wei: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  7. Zi Fang: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  8. Xiaoyan Wu: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  9. Yanan Wang: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  10. Yongbiao Xue: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. ORCID
  11. Biyao Zhang: National Genomics Data Center & CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China. ORCID
PMID: 38934055 DOI: 10.1111/nph.19928

Abstract

Climate warming poses a significant threat to global crop production and food security. However, our understanding of the molecular mechanisms governing thermoresponsive development in crops remains limited. Here we report that the auxiliary subunit of N-terminal acetyltransferase A (NatA) in rice OsNAA15 is a prerequisite for rice thermoresponsive growth. OsNAA15 produces two isoforms OsNAA15.1 and OsNAA15.2, via temperature-dependent alternative splicing. Among the two, OsNAA15.1 is more likely to form a stable and functional NatA complex with the potential catalytic subunit OsNAA10, leading to a thermoresponsive N-terminal acetylome. Intriguingly, while OsNAA15.1 promotes plant growth under elevated temperatures, OsNAA15.2 exhibits an inhibitory effect. We identified two glycolate oxidases (GLO1/5) as major substrates from the thermoresponsive acetylome. These enzymes are involved in hydrogen peroxide (HO) biosynthesis via glycolate oxidation. N-terminally acetylated GLO1/5 undergo their degradation through the ubiquitin-proteasome system. This leads to reduced reactive oxygen species (ROS) production, thereby promoting plant growth, particularly under high ambient temperatures. Conclusively, our findings highlight the pivotal role of N-terminal acetylation in orchestrating the glycolate-mediated ROS homeostasis to facilitate thermoresponsive growth in rice.

Keywords

N���terminal acetylation ROS glycolate oxidase rice temperature thermoresponsive growth

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Grants

  1. 32172011/National Natural Science Foundation of China
  2. XDA24010302/Strategic Priority Research Program of the Chinese Academy of Sciences

MeSH Term

Oryza
Acetylation
Reactive Oxygen Species
Homeostasis
Plant Proteins
Temperature
Glycolates
Hydrogen Peroxide
Proteolysis
Gene Expression Regulation, Plant
Alcohol Oxidoreductases

Chemicals

Reactive Oxygen Species
Plant Proteins
Glycolates
glycolic acid
Hydrogen Peroxide
glycollate oxidase
Alcohol Oxidoreductases
Journal Article

Links to CNCB-NGDC Resources

BioProject: PRJCA020807 (crop acclimation to heat stress)
OMIX: OMIX005150 (N-terminal acetylome of Osnaa15 and WT in rice)
Article has an altmetric score of 3

Word Cloud

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