| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
cox2
|
476 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 13.50% | Poor | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 2.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
cox3
|
1062 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 82.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 57.50% | Medium | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
cox3
|
894 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 88.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 68.00% | High | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
cox3
|
906 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 84.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 62.00% | High | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad1
|
2685 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 80.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 34.00% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad2A
|
461 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 48.50% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 25.00% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad2A
|
59 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 44.50% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 28.50% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad2B
|
731 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 62.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 34.00% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
149 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 47.50% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 7.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
212 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 55.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 9.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
213 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 51.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 10.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
250 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 77.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 8.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
254 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 50.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 11.50% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
347 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 68.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 10.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
352 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 85.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 4.50% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
64 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 50.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 15.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad3
|
83 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 53.50% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 12.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad4
|
158 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 90.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 55.00% | Medium | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad4
|
2701 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 91.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 71.00% | High | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad4
|
2756 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 90.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 76.00% | High | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad4
|
437 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 85.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 47.50% | Medium | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad4
|
449 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 87.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 47.50% | Medium | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad4
|
74 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 90.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 55.00% | Medium | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad6
|
103 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 42.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 10.00% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad6
|
53 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 62.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 18.50% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad6
|
88 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 63.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 22.50% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad6
|
89 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 73.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 23.50% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad7
|
1172 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
S=>N |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 60.80% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 28.20% | Low | Decreased | 29580769 | | Columbia (Col-0) | pBLX:BLX/blx/- | Complementation | For complementation assay, we generated the TOPO_BLX_P-G-3′UTR construct containing the coding sequence of BLX (AT2g15690), the 2-kb region upstream of the ATG codon and the 267-bp region downstream o | Homozygous | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 58.40% | Medium | Restored | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad7
|
2239 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 65.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 32.50% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad7
|
2370 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 52.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 20.00% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
nad7
|
3848 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 39.00% | Low | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 26.00% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
rps12
|
146 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 54.00% | Medium | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 26.00% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
rps3
|
1334 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 5.50% | Poor | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
rps3
|
1344 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 20.00% | Low | None | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
rps3
|
1352 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 78.50% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 38.50% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
rps3
|
1470 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 71.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 23.50% | Low | Decreased | 29580769 |
|
| Arabidopsis thaliana |
AT2G15690 |
Mitochondrion |
rps3
|
1571 |
CDS |
NA |
NC_001284.2 |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 78.00% | High | None | 29580769 | | N756159 | blx/- | A T-DNA insertion | A T-DNA insertion mutant N756159 (blx) from the Nottingham Arabidopsis Stock Centre (NASC) (Scholl et al., 2000). The T-DNA was inserted in the site corresponding to the fourth PPR repeat of BLX. | Homozygous | Mutant embryos in blx/+ siliques were arrested before the early heart stage with irregular division patterns. In the same blx/+ siliques, when embryos in the wild-type-like normal ovules developed into the early heart embryo stage at 3 days after pollination (DAP), embryos in the aborted ovules (n = 178) were still at the sequential stages containing one- to eight-cell embryos. In the blx/- mutant endosperm, the nuclei demonstrated a messy distribution pattern, and less radial microtubules were formed within each NCD. When most of the wild-type endosperm cells completed the cellularization, the initiation of endosperm cellularization could not be observed in the blx/- embryo sacs. | Ovule | NA | strand- and transcript-specific RNA-seq method (STS-PCRseq) | 41.00% | Medium | Decreased | 29580769 |
|
