Plant Editosome Database
a curated database of RNA editosome in plants

ISE2 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: ISE2
Synonym: EMB25, EMBRYO DEFECTIVE 25, INCREASED SIZE EXCLUSION LIMIT 2, PDE317, PIGMENT DEFECTIVE 317
Description: Encodes a chloroplast RNA helicase required for multiple chloroplast RNA processing steps in Arabidopsis thaliana.
Protein Family: RNA Helicase
Subclass: NA
Construct Structure: NA
Gene ID & Species: AT1G70070 (Arabidopsis thaliana)
Edited Gene(s): clpP    rps14    petL    rpoA    rpoB
Editing Type(s): C-to-U (24)
Publication(s): [1] The chloroplast RNA helicase ISE2 is required for multiple chloroplast RNA processing steps in Arabidopsis thaliana, The Plant Journal, 2017. [PMID=28346704]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G70070 Chloroplast clpP 559 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ise2-2 homozygousvar2-2NANANAVariegation mutantLeaf12 daysDirect Sequencing of PCR Products63.00%HighDecreased28346704
ise2-2 homozygousISE2-GFP (ISE2-OE)ComplementationNARescued plantsNALeaf12 daysDirect Sequencing of PCR Products77.30%HighRestored28346704
ise2-2 homozygousISE2-GFP transgene (ISE2-SUP)ComplementationNACosuppression; Reduced accumulation of the transgene transcript and No ISE2-GFP ProteinLeaves developed chlorosisLeaf12 daysDirect Sequencing of PCR Products25.80%LowDecreased28346704
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf12 daysDirect Sequencing of PCR Products83.10%HighNone28346704
Arabidopsis thaliana AT1G70070 Chloroplast petL 5 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ise2-2 homozygousvar2-2NANANAVariegation mutantLeaf12 daysDirect Sequencing of PCR Products64.00%HighDecreased28346704
ise2-2 homozygousISE2-GFP (ISE2-OE)ComplementationNARescued plantsNALeaf12 daysDirect Sequencing of PCR Products87.80%HighRestored28346704
ise2-2 homozygousISE2-GFP transgene (ISE2-SUP)ComplementationNACosuppression; Reduced accumulation of the transgene transcript and No ISE2-GFP ProteinLeaves developed chlorosisLeaf12 daysDirect Sequencing of PCR Products48.50%MediumDecreased28346704
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf12 daysDirect Sequencing of PCR Products88.90%HighNone28346704
Arabidopsis thaliana AT1G70070 Chloroplast rpoA 200 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ise2-2 homozygousvar2-2NANANAVariegation mutantLeaf12 daysDirect Sequencing of PCR Products64.10%HighDecreased28346704
ise2-2 homozygousISE2-GFP (ISE2-OE)ComplementationNARescued plantsNALeaf12 daysDirect Sequencing of PCR Products90.50%HighRestored28346704
ise2-2 homozygousISE2-GFP transgene (ISE2-SUP)ComplementationNACosuppression; Reduced accumulation of the transgene transcript and No ISE2-GFP ProteinLeaves developed chlorosisLeaf12 daysDirect Sequencing of PCR Products40.50%MediumDecreased28346704
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf12 daysDirect Sequencing of PCR Products88.20%HighNone28346704
Arabidopsis thaliana AT1G70070 Chloroplast rpoB 338 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ise2-2 homozygousvar2-2NANANAVariegation mutantLeaf12 daysDirect Sequencing of PCR Products93.80%HighSimilar28346704
ise2-2 homozygousISE2-GFP (ISE2-OE)ComplementationNARescued plantsNALeaf12 daysDirect Sequencing of PCR Products97.60%HighRestored28346704
ise2-2 homozygousISE2-GFP transgene (ISE2-SUP)ComplementationNACosuppression; Reduced accumulation of the transgene transcript and No ISE2-GFP ProteinLeaves developed chlorosisLeaf12 daysDirect Sequencing of PCR Products72.30%HighDecreased28346704
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf12 daysDirect Sequencing of PCR Products94.10%HighNone28346704
Arabidopsis thaliana AT1G70070 Chloroplast rpoB 551 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ise2-2 homozygousvar2-2NANANAVariegation mutantLeaf12 daysDirect Sequencing of PCR Products98.80%HighSimilar28346704
ise2-2 homozygousISE2-GFP (ISE2-OE)ComplementationNARescued plantsNALeaf12 daysDirect Sequencing of PCR Products97.70%HighRestored28346704
ise2-2 homozygousISE2-GFP transgene (ISE2-SUP)ComplementationNACosuppression; Reduced accumulation of the transgene transcript and No ISE2-GFP ProteinLeaves developed chlorosisLeaf12 daysDirect Sequencing of PCR Products76.20%HighDecreased28346704
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf12 daysDirect Sequencing of PCR Products95.70%HighNone28346704
Arabidopsis thaliana AT1G70070 Chloroplast rps14 149 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ise2-2 homozygousvar2-2NANANAVariegation mutantLeaf12 daysDirect Sequencing of PCR Products66.20%HighSimilar28346704
ise2-2 homozygousISE2-GFP (ISE2-OE)ComplementationNARescued plantsNALeaf12 daysDirect Sequencing of PCR Products86.10%HighRestored28346704
ise2-2 homozygousISE2-GFP transgene (ISE2-SUP)ComplementationNACosuppression; Reduced accumulation of the transgene transcript and No ISE2-GFP ProteinLeaves developed chlorosisLeaf12 daysDirect Sequencing of PCR Products42.20%MediumDecreased28346704
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf12 daysDirect Sequencing of PCR Products69.10%HighNone28346704
Last update: Jul 2021 (version 1.0)