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MORF9 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: MORF9
Synonym: MULTIPLE ORGANELLAR RNA EDITING FACTOR 9; RIP9
Description: MORF2-MORF9 heterodimer is the core members of editosomes in chloroplasts, while they are not responsible for RNA editing site recognition; MORF9-mediated RNA editing is tissue-dependent and the resultant organelle proteomes are pertinent to the specific tissue functions; Loss of MORF9 function impaired PSII efficiency, NDH activity, and carbohydrate production, rapidly promoted nuclear gene expression including sucrose transporter and sugar/energy responsive genes, and attenuated root growth under sugar starvation conditions; Sugar repletion increased MORF9 and MORF2 expression in wild-type seedlings and reduced RNA editing of matK-706, accD-794, ndhD-383 and ndhF-290 in the morf9 mutant; RNA editing efficiency of ndhD-383 and ndhF-290 sites was diminished in the gin2/morf9 double mutants, and that of matK-706, accD-794, ndhD-383 and ndhF-290 sites were significantly diminished in the snrk1/morf9 double mutants; In contrast, overexpressing HXK1 or SnRK1 promoted RNA editing rate of matK-706, accD-794, ndhD-383 and ndhF-290 in leaves of morf9 mutants, suggesting that HXK1 partially impacts MORF9 mediated ndhD-383 and ndhF-290 editing, while SnRK1 may only affect MORF9-mediated ndhF-290 site editing
Protein Family: Multiple organellar RNA editing factor (MORF)
Subclass: NA
Physical Interaction: OsSLA4; DUA1
Construct Structure: NA
Gene ID & Species: LOC_Os08g04450 (Oryza sativa)
Edited Gene(s): atpA    ndhA    ndhD    ndhG    rpl2    rps8    rpoC2    rps14    ycf3    ndhB    rpoB
Editing Type(s): C-to-U (54)
Publication(s): [1] OsMORF9 is necessary for chloroplast development and seedling survival in rice, Plant Sci, 2021. [doi:10.1016/j.plantsci.2021.110907]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_Os08g04450 Chloroplast atpA 1148 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhA 1070 CDS NA NA NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products73.91%HighNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products20.00%LowDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products17.86%PoorDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 467 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products40.54%MediumDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products32.43%LowDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 586 CDS NA NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products20.69%LowDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products28.57%LowDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 611 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products30.77%LowDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products17.95%PoorDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 704 CDS NA NA NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products91.67%HighNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products44.68%MediumDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products32.50%LowDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 737 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products62.22%HighNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products11.76%PoorDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 830 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products32.00%LowDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products83.67%HighDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhB 836 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products35.42%LowDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products77.78%HighDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhD 878 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products80.00%HighNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast ndhG 10 CDS NA NA NA NA C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products57.14%MediumDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products55.56%MediumDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast rpl2 2 CDS NA NA NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products50.00%MediumNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast rpoB 467 CDS NA NA NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast rpoB 545 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products80.77%HighNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products16.67%PoorDecreased33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products19.61%PoorDecreased33902846
Oryza sativa LOC_Os08g04450 Chloroplast rpoC2 4106 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast rps14 80 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast rps8 182 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Oryza sativa LOC_Os08g04450 Chloroplast ycf3 185 CDS NA NA NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareWTControlControlNo mutantNormalLeafNASequencing RT-PCR products100.00%CompleteNone33902846
Nipponbareosmorf9-1DeletionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Nipponbareosmorf9-2InsertionSequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site.KnockoutPhenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants.LeafNASequencing RT-PCR products0.00%UneditedAbsent33902846
Last update: Sep 2026 (version 2.0)