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PPR278 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: PPR278
Synonym: NA
Description: Loss-function of PPR278 led to a lower germination rate and other defects at the seedling stage, as well as smaller kernels compared to the wild type; PPR278 was expressed in all investigated tissues; PPR278 is involved in the splicing of two mitochondrial transcripts (nad2 intron 4 and nad5 introns 1 and 4), as well as RNA editing of C-to-U sites in 10 mitochondrial transcripts
Protein Family: PPR
Subclass: P
Physical Interaction: NA
Construct Structure: P
Gene ID & Species: Zm00001d015156 (Zea mays)
Edited Gene(s): atp1    atp4    atp8    cox3    nad3    rpl16    rps2A    rps2B    rps12    ccmFn
Editing Type(s): C-to-U (273)
Publication(s): [1] Maize PPR278 Functions in Mitochondrial RNA Splicing and Editing., International journal of molecular sciences, 2022. [PMID=35328469]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays Zm00001d015156 Mitochondrion atp1 1178 CDS B73 (V4) B73 (V4) NA NA C-to-U UUC=>UUU S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products70.51%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp1 1292 CDS B73 (V4) B73 (V4) NA NA C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products60.77%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp1 1490 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products80.99%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp1 1499 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products70.83%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp1 30 CDS B73 (V4) B73 (V4) NA NA C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products34.62%LowNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp4 118 CDS B73 (V4) B73 (V4) NA NA C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp4 360 CDS B73 (V4) B73 (V4) NA NA C-to-U UGC=>UGU C=>C Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products36.92%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp4 407 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products19.80%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp4 428 CDS B73 (V4) B73 (V4) NA NA C-to-U ACU=>AUU T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products38.55%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp4 56 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion atp4 59 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products7.78%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp4 71 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products84.25%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products27.07%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp4 76 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products22.15%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp4 89 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products27.59%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp8 123 CDS B73 (V4) B73 (V4) NA NA C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products85.71%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products35.96%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products63.92%HighDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp8 200 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products85.11%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products65.66%HighDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products61.76%HighDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp8 436 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products68.97%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products60.78%HighSimilar35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products41.67%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp8 437 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products81.82%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products63.16%HighDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products64.00%HighDecreased35328469
Zea mays Zm00001d015156 Mitochondrion atp8 58 CDS B73 (V4) B73 (V4) NA NA C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products82.05%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products58.90%MediumDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products45.16%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1325 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1342 CDS B73 (V4) B73 (V4) NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1357 CDS B73 (V4) B73 (V4) NA NA C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 137 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1375 CDS B73 (V4) B73 (V4) NA NA C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1408 CDS B73 (V4) B73 (V4) NA NA C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1469 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1489 CDS B73 (V4) B73 (V4) NA NA C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1493 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1505 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1540 CDS B73 (V4) B73 (V4) NA NA C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1553 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1588 CDS B73 (V4) B73 (V4) NA NA C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 1709 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly -5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 176 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 181 CDS B73 (V4) B73 (V4) NA NA C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 190 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 287 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 302 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 401 CDS B73 (V4) B73 (V4) NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 410 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 417 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 743 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 752 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 76 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>UUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 77 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>UUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 790 CDS B73 (V4) B73 (V4) NA NA C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 812 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 824 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion ccmFn 839 CDS B73 (V4) B73 (V4) NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANANone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR productsNANAAbsent35328469
Zea mays Zm00001d015156 Mitochondrion cox3 245 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 257 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products45.98%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 289 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products60.32%HighDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 311 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 314 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 413 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products52.70%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 422 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products41.18%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 527 CDS B73 (V4) B73 (V4) NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products17.14%PoorDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products67.95%HighDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 566 CDS B73 (V4) B73 (V4) NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products76.70%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products62.35%HighSimilar35328469
Zea mays Zm00001d015156 Mitochondrion cox3 69 CDS B73 (V4) B73 (V4) NA NA C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products34.83%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion cox3 754 CDS B73 (V4) B73 (V4) NA NA C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products70.00%HighDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 137, 138 CDS B73 (V4) B73 (V4) NA NA C-to-U UCC=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products88.37%, 3.High, PoorNone, None35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products32.20%, 0.Low, UnediDecreased,35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products26.67%, 0.Low, UnediDecreased,35328469
Zea mays Zm00001d015156 Mitochondrion nad3 146 CDS B73 (V4) B73 (V4) NA NA C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products80.37%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products14.29%PoorDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion nad3 185 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products77.78%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products41.67%MediumDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products33.33%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 190 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products73.68%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products23.94%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products17.35%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 208, 209 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>UUU P=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products76.54%, 78High, HighNone, None35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products26.32%, 35Low, LowDecreased,35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products18.75%, 22Poor, LowDecreased,35328469
Zea mays Zm00001d015156 Mitochondrion nad3 215 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products66.67%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products23.08%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion nad3 230 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products22.73%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products16.67%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 247 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products69.70%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products26.15%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products18.37%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 251 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products23.08%LowNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products23.19%LowSimilar35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products11.34%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 275 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products71.30%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products20.83%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products13.16%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 317 CDS B73 (V4) B73 (V4) NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products40.79%MediumSimilar35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products33.60%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 344 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products65.35%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products37.21%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products33.60%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 349 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products40.45%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products35.00%LowSimilar35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products35.14%LowSimilar35328469
Zea mays Zm00001d015156 Mitochondrion nad3 39 CDS B73 (V4) B73 (V4) NA NA C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products38.95%LowNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products24.64%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products33.33%LowSimilar35328469
Zea mays Zm00001d015156 Mitochondrion nad3 44 CDS B73 (V4) B73 (V4) NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products56.63%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products44.44%MediumDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumSimilar35328469
Zea mays Zm00001d015156 Mitochondrion nad3 5 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products70.65%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products55.36%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion nad3 61, 62 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products56.25%, 44Medium, MeNone, None35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products32.93%, 30Low, LowDecreased,35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products39.84%, 30Low, LowDecreased,35328469
Zea mays Zm00001d015156 Mitochondrion nad3 80 CDS B73 (V4) B73 (V4) NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products37.50%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products31.51%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rpl16 228 CDS B73 (V4) B73 (V4) NA NA C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products53.42%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products23.08%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products30.00%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps12 196 CDS B73 (V4) B73 (V4) NA NA C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products61.22%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products39.58%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products42.11%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps12 221 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products66.00%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products34.88%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products38.78%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps12 269 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products55.77%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products34.69%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products39.02%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps12 284 CDS B73 (V4) B73 (V4) NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products65.52%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products40.74%MediumDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products43.40%MediumDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps12 289 CDS B73 (V4) B73 (V4) NA NA C-to-U CGU=>UCU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products66.67%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products20.83%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products17.65%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps12 71 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products50.00%MediumNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products16.67%PoorDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products20.58%LowDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps2A 200 CDS B73 (V4) B73 (V4) NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products20.00%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products15.79%PoorDecreased35328469
Zea mays Zm00001d015156 Mitochondrion rps2A 449 CDS B73 (V4) B73 (V4) NA NA C-to-U GCA=>GUA A=>V Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products84.31%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products25.71%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion rps2A 514 CDS B73 (V4) B73 (V4) NA NA C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products30.56%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion rps2A 541 CDS B73 (V4) B73 (V4) NA NA C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products23.33%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion rps2A 548 CDS B73 (V4) B73 (V4) NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products94.34%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products22.22%LowDecreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products0.00%UneditedAbsent35328469
Zea mays Zm00001d015156 Mitochondrion rps2B 550 CDS B73 (V4) B73 (V4) NA NA C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernel12 days after pollination (DAP)Directly sequencing RT-PCR products63.83%HighNone35328469
NAppr278-1The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveSmaller seeds; Only ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stage; The 100-kernel weight of ppr278 mutant plants was significantly lower than that of WTKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteIncreased35328469
NAppr278-2The maize (Zea mays L.) ppr278-1 and ppr278-2 mutants were obtained from the authors' EMS-based mutant library.NARecessiveOnly ~5% of mutant kernels could germinate normally on average and grew to the seedling stage; The vigor of mutant seedlings was notably decreased, and homozygous mutant seedlings did not survive to the reproductive stageKernel12 days after pollination (DAP)Directly sequencing RT-PCR products100.00%CompleteIncreased35328469
Last update: Feb 2026 (version 2.0)